Inhibition of HIV-1 replication by simian restriction factors, TRIM5α and APOBEC3G

被引：27

作者：

Sakuma, R. ^{[1
]}

Noser, J. A. ^{[1
]}

Ohmine, S. ^{[1
]}

Ikeda, Y. ^{[1
]}

机构：

[1] Mayo Clin, Coll Med, Program Mol Med, Rochester, MN 55905 USA

来源：

GENE THERAPY | 2007年 / 14卷 / 02期

关键词：

HIV-1; TRIM5; alpha; APOBEC3G; restriction factor; innate immunity;

D O I：

10.1038/sj.gt.3302852

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Old World monkey TRIM5 alpha targets incoming human immunodeficiency virus type 1 ( HIV-1) viral capsid, whereas the apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like ( APOBEC) 3 family hypermutate/degrade viral sequences. Here, we show the potentials of simian TRIM5 alpha and APOBEC3G as therapeutic sequences for AIDS gene therapy. Both rhesus and African green monkey ( agm) TRIM5 alpha efficiently restrict HIV-1 vectors with divergent Gag from different HIV-1 subtypes. Human T cells genetically engineered to express agm-TRIM5 alpha block or delay HIV-1 replication. Although agm-APOBEC3G expression alone only transiently suppresses HIV-1 replication, co-expression of agm-APOBEC3G and agm-TRIM5 alpha successfully block the virus replication for more than 5 weeks.

引用

页码：185 / 189

页数：5

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