Comparative Analysis of Genetic Diversity among Bt Cotton Genotypes Using EST-SSR, ISSR and Morphological Markers

被引：0
作者：
Ashraf, J. [1 ]
Malik, W. [1 ]
Iqbal, M. Z. [2 ]
Khan, A. Ali [3 ]
Qayyum, A. [1 ]
Noor, E. [1 ]
Abid, M. A. [1 ]
Cheema, H. M. Naseer [3 ]
Ahmad, M. Q. [1 ]
机构：
[1] Bahauddin Zakariya Univ, Fac Agr Sci & Technol, Dept Plant Breeding & Genet, Multan, Pakistan
[2] AARI, Agr Biotechnol Res Inst, Faisalabad, Pakistan
[3] Univ Agr Faisalabad, Dept Plant Breeding & Genet, Faisalabad, Pakistan
来源：
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY | 2016年 / 18卷 / 02期
关键词：
Confusion probability; Expected heterozygosity; Marker Index; Population structure analysis; Polymorphic information contents; DISCRIMINATING CAPACITY; RAPD MARKERS; GOSSYPIUM; MICROSATELLITES; POPULATION; ACCESSIONS; VARIETIES; AFLP; L;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Robust information of genetic diversity among Bt cotton genotypes is still lacking and availability of several marker systems has prompted us to compare their utility for the detection of genetic diversity. Here, we report the comparative efficiency of morphological and molecular markers (EST-SSRs and ISSRs) in determining the genetic diversity among 30 Pakistani Bt cotton genotypes. Three different dendrograms based on 20 EST-SSRs, 13 ISSRs and 20 morphological markers divided the 30 Bt cotton genotypes into five, six, and three clusters, respectively. EST-SSRs and ISSRs revealed 0.73-1.00 and 0.77-0.97 genetics similarity among Bt cotton genotypes which indicated low level of genetic diversity. Further population structure analysis showed extensive allelic admixture among Bt cotton cultivars and identified three (EST-SSRs) and six (ISSRs) subgroups. The MGHES-31 (EST-SSRs) and UBC-807 and UBC-815 (ISSRs) showed maximum values of Polymorphic Information Contents (PIC) and Dj and had low value of Cj. The comparison of two marker systems showed that EST-SSRs had high value of Expected heterozygosity (H-ep) and low value of Marker Index (MI) as compared to ISSRs. In conclusion, high level of genetic similarity among Bt cotton genotypes compel the plant breeders for the introduction of divers/exotic genotypes in their breeding program and marker discriminating indices could be a potential tool for selection of particular marker system to unveil the genetic difference.
引用
收藏
页码：517 / 531
页数：15
相关论文
共 33 条
  • [1] Alvarez I, 2006, EVOLUTION, V60, P505, DOI 10.1111/j.0014-3820.2006.tb01132.x
  • [2] OPTIMIZING PARENTAL SELECTION FOR GENETIC-LINKAGE MAPS
    ANDERSON, JA
    CHURCHILL, GA
    AUTRIQUE, JE
    TANKSLEY, SD
    SORRELLS, ME
    [J]. GENOME, 1993, 36 (01) : 181 - 186
  • [3] Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive
    Belaj, A
    Satovic, Z
    Cipriani, G
    Baldoni, L
    Testolin, R
    Rallo, L
    Trujillo, I
    [J]. THEORETICAL AND APPLIED GENETICS, 2003, 107 (04) : 736 - 744
  • [4] Dalamu Dalamu, 2012, Australian Journal of Crop Science, V6, P261
  • [5] DEYNZE AV, 2009, BMC PLANT BIOL, V9, P125
  • [6] Dongre Ashok, 2004, Indian Journal of Biotechnology, V3, P388
  • [7] Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites
    Kantartzi, Stella K.
    Ulloa, Mauricio
    Sacks, Erik
    Stewart, James McD.
    [J]. GENETICA, 2009, 136 (01) : 141 - 147
  • [8] A modified mini-prep method for economical and rapid extraction of genomic DNA in plants
    Khan I.A.
    Awan F.S.
    Ahmad A.
    Khan A.A.
    [J]. Plant Molecular Biology Reporter, 2004, 22 (1) : 89a - 89e
  • [9] Genetic relationships and discrimination of ten influential Upland cotton varieties using RAPD markers
    Lu, HJ
    Myers, GO
    [J]. THEORETICAL AND APPLIED GENETICS, 2002, 105 (2-3) : 325 - 331
  • [10] Lukonge K., 2007, AFR CROP SCI C P, V8, P773
1234