Photoencapsulated-BMP2 in visible light-cured thiol-acrylate hydrogels for craniofacial bone tissue engineering

被引：8

作者：

Aghali, Arbi ^{[1
,2
]}

Arman, Huseyin E. ^{[3
]}

机构：

[1] Mayo Clin, Dept Physiol & Biomed Engn, Rochester, MN 55902 USA

[2] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47908 USA

[3] Indiana Univ Sch Med, Dept Med, Indianapolis, IN 46202 USA

来源：

REGENERATIVE MEDICINE | 2020年 / 15卷 / 09期

关键词：

biodegradation; craniofacial bone; PEGDA hydrogels; stem cells; thiol-acrylate; tissue engineering; MESENCHYMAL STROMAL CELLS; STEM-CELLS; OSTEOGENIC DIFFERENTIATION; MORPHOGENETIC PROTEIN-2; MARROW; BIOMATERIALS; REGENERATION; DELIVERY; FATE;

D O I：

10.2217/rme-2020-0062

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Aim: The study aimed to examine the impact of crosslinking BMP2 in biodegradable visible light-cured thiol-acrylate hydrogels. Materials & methods: BMP2 was photoencapsulated in 10 wt% PEG-diacrylate hydrogels with or without immortalized mouse bone marrow stromal cells (BMSC). Results & conclusion: Photoencapsulated-BMSC with BMP2 (BMBMP2) showed a significantly (p < 0.05) increased level in metabolic activity, by 54.61%, compared with photoencapsulated-BMSC at day 3. Furthermore, BMBMP2 groups showed significantly increased levels in ALP activity compared with BMSC at days, 1, 3, 7 (p < 0.01) and 10 (p < 0.05). This study shows promising results photoencapsulating BMP2 in thiol-acrylate hydrogels for craniofacial bone tissue engineering applications.

引用

页码：2099 / 2113

页数：15

共 63 条

[1]

Aghali A.M., 2017, Orthopedic Biomaterials, P225, DOI [10.1007/978-3-319-73664-8_9, 10.1007/978-3-319-73664-89, DOI 10.1007/978-3-319-73664-89]

[2] Hydrogel: Preparation, characterization, and applications: A review [J].

Ahmed, Enas M. .

JOURNAL OF ADVANCED RESEARCH, 2015, 6 (02) :105-121

[3] Pediatric mesenchymal stromal cells therapy: an update [J].

An, Qi ;

Fan, Conghai ;

Xu, Shumei .

MINERVA PEDIATRICA, 2018, 70 (04) :396-402

[4]

Annabi N, 2010, TISSUE ENG PART B-RE, V16, P371, DOI [10.1089/ten.teb.2009.0639, 10.1089/ten.TEB.2009.0639]

[5]

[Anonymous], 2018, APPL SCI, DOI DOI 10.16562/j.cnki.0256-1492.2018.05.001

[6] A Perspective on Stem Cells as Biological Systems that Produce Differentiated Osteoblasts and Odontoblasts [J].

Ariffin, Shahrul Hisham Zainal ;

Manogaran, Thanaletchumi ;

Abidin, Intan Zarina Zainol ;

Wahab, Rohaya Megat Abdul ;

Senafi, Sahidan .

CURRENT STEM CELL RESEARCH & THERAPY, 2017, 12 (03) :247-259

[7]

Beederman M., 2013, J BIOMEDICAL SCI ENG, V6, P32, DOI 10.4236/jbise.2013.68A1004

[8]

Bhat S., 2013, BIOMATTER, V3, DOI [10.4161/biom.247172-s2.0-85003052527, DOI 10.3126/BJ.V3I1.7508]

[9] Paclitaxel-releasing mesenchymal stromal cells inhibit the growth of multiple myeloma cells in a dynamic 3D culture system [J].

Bonomi, Arianna ;

Steimberg, Nathalie ;

Benetti, Anna ;

Berenzi, Angiola ;

Alessandri, Giulio ;

Pascucci, Luisa ;

Boniotti, Jennifer ;

Cocce, Valentina ;

Sordi, Valeria ;

Pessina, Augusto ;

Mazzoleni, Giovanna .

HEMATOLOGICAL ONCOLOGY, 2017, 35 (04) :693-702

[10] Strategies for MSC expansion and MSC-based microtissue for bone regeneration [J].

Bunpetch, Varitsara ;

Zhang, Zhi-Yong ;

Zhang, Xiaoan ;

Han, Shan ;

Pan Zongyou ;

Wu, Haoyu ;

Ouyang Hong-Wei .

BIOMATERIALS, 2019, 196 :67-79

← 1 2 3 4 5 6 7 →