PIP1 plasma membrane aquaporins in tobacco: From cellular effects to function in plants

被引：273

作者：

Siefritz, F

Tyree, MT

Lovisolo, C

Schubert, A

Kaldenhoff, R ^{[1
]}

机构：

[1] Univ Wurzburg, Dept Plant Physiol & Biophys, D-97082 Wurzburg, Germany

[2] USDA, Forest Serv, Aiken Forestry Sci Lab, Burlington, VT 05402 USA

[3] Univ Turin, Dept Arboriculture & Pomol, I-10095 Grugliasco, Italy

[4] Italian Natl Res Council, Grapevine Breeding & Biol Ctr, I-10095 Grugliasco, Italy

来源：

PLANT CELL | 2002年 / 14卷 / 04期

关键词：

D O I：

10.1105/tpc.000901

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular functions of several aquaporins are well characterized (e.g., by analysis of aquaporin-expressing Xenopus oocytes). However, their significance in the physiology of water transport in multicellular organisms remains uncertain. The tobacco plasma membrane aquaporin NtAQP1 was used to elucidate this issue. By comparing antisense plants that were inhibited in NtAQP1 expression with control plants, we found evidence for NtAQP1 function in cellular and whole-plant water relations. The consequences of a decrease in cellular water permeability were determined by measurement of transpiration rate and stem and leaf water potential as well as growth experiments under extreme soil water depletion. Plants impaired in NtAQP1 expression showed reduced root hydraulic conductivity and lower water stress resistance. In conclusion, our results emphasize the importance of symplastic aquaporin-mediated water transport in whole-plant water relations.

引用

页码：869 / 876

页数：8

共 19 条

[1] Aquaporin null phenotypes: The importance of classical physiology [J].