A novel set of 223 EST-SSR markers in Casuarina L. ex Adans.: polymorphisms, cross-species transferability, and utility for commercial clone genotyping

被引：16
|
作者：
Xu, Xiuyu [1 ,2 ]
Zhou, Changpin [3 ]
Zhang, Yong [3 ]
Zhang, Weiqiang [1 ]
Gan, Xianhua [1 ]
Zhang, Huaxin [2 ]
Guo, Yong [4 ]
Gan, Siming [3 ]
机构：
[1] Guangdong Acad Forestry, Guangdong Key Lab Forest Silviculture & Utilizat, Guangzhou 510520, Guangdong, Peoples R China
[2] Chinese Acad Forestry, Res Ctr State Forestry Adm Saline & Alkali Land, Xiangshan Rd, Beijing 100091, Peoples R China
[3] Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab State Forestry Adm Trop Forestry Res, Guangzhou 510520, Guangdong, Peoples R China
[4] Guangzhou Geneunion Biotechnol Co Ltd, Ruifeng Ctr, Room 617,Bldg 2, Guangzhou 510530, Guangdong, Peoples R China
来源：
TREE GENETICS & GENOMES | 2018年 / 14卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Casuarina; Expressed sequence tag (EST); Simple sequence repeat (SSR); Polymorphism; Transferability; Clone genotyping; MICROSATELLITE MARKERS; GENETIC DIVERSITY; ISSR;
D O I：
10.1007/s11295-018-1246-0
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
Simple sequence repeat (SSR) markers are very useful for genetic applications in plants, but SSR resource for the important tree genus Casuarina L. ex Adans. is still limited. In this study, we report a novel set of 223 SSR markers in Casuarina developed from expressed sequence tag (EST) resource of GenBank. The 223 EST-SSR markers were polymorphic among 10 unrelated individuals of C. equisetifolia L. Johnson, with the number of alleles per locus (N-a), observed heterozygosity (H-o), expected heterozygosity (He), and polymorphic information content (PIC) averaging at 5.5, 0.72, 0.86, and 0.63, respectively. The rates of cross-species transferability ranged from 96.9% (C. glauca Sieber ex Sprengel) through 97.8% (C. cunninghamiana Miquel) to 99.1% (C. junghuhniana Miquel). Fifty-five C. equisetifolia clones widely planted in China were successfully genotyped with a subset of 20 EST-SSRs. These newly developed markers will have a great potential for genetic and breeding applications in Casuarina species and related taxa.
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页数：8
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