Long term betaine supplementation regulates genes involved in lipid and cholesterol metabolism of two muscles from an obese pig breed

被引：31

作者：

Albuquerque, A. ^{[1
]}

Neves, Jose A. ^{[2
]}

Redondeiro, M. ^{[1
]}

Laranjo, M. ^{[1
]}

Felix, M. R. ^{[3
]}

Freitas, Amadeu ^{[2
]}

Tirapicos, Jose L. ^{[4
]}

Martins, Jose M. ^{[2
]}

机构：

[1] Univ Evora, ICAAM, Nucleo Mitra, Ap 94, P-7006554 Evora, Portugal

[2] Univ Evora, ICAAM, Dept Zootecnia, Polo Mitra, Ap 94, P-7002554 Evora, Portugal

[3] Univ Evora, Dept Fitotecnia, ICAAM, P-7002554 Evora, Portugal

[4] Univ Evora, Dept Med Vet, ICAAM, P-7002554 Evora, Portugal

来源：

MEAT SCIENCE | 2017年 / 124卷

关键词：

Betaine; Longissimus lumborum; Biceps femoris; Intramuscular fat and cholesterol content; Gene expression; Alentejano pig; FATTY-ACID-COMPOSITION; PORCINE ADIPOSE-TISSUE; INTRAMUSCULAR FAT; LARGE WHITE; EXPRESSION; PROTEIN; ADIPOCYTE; QUALITY; DIFFERENTIATION; TRANSCRIPTION;

D O I：

10.1016/j.meatsci.2016.10.012

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study evaluates the effects of betaine supplementation (1 g kg(-1) for 20 weeks) on the regulation of genes involved in lipid and cholesterol metabolism of Longissimus lumborum and Biceps femoris from obese Alentejano pigs. Betaine supplementation led to an increase in total cholesterol in both muscles, complementing results previously published indicating a significant increase on the intramuscular lipid content. The expression of twelve genes involved in lipogenesis, lipolysis/FA oxidation, FA transport, and cholesterol metabolism, as well as two transcription factors were also evaluated. Genes related to lipid and cholesterol synthesis plus FA transport were consistently up-regulated in both muscles of betaine fed pigs. On the other hand, genes related to lipolysis/FA oxidation were not affected or down-regulated by betaine supplementation. Our data suggest that the underlying mechanism regulating IMF and cholesterol accumulation in Alentejano pigs supplemented with betaine is associated with the up-regulation of genes involved in lipid synthesis, FA transport, and cholesterol synthesis. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：25 / 33

页数：9

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