Gold-Nanoparticle-Based Colorimetric Discrimination of Cancer-Related Point Mutations with Picomolar Sensitivity

被引：99

作者：

Valentini, Paola ^{[1
]}

Fiammengo, Roberto ^{[1
]}

Sabella, Stefania ^{[1
]}

Gariboldi, Manuela ^{[2
,3
]}

Maiorano, Gabriele ^{[1
]}

Cingolani, Roberto ^{[4
]}

Pompa, Pier Paolo ^{[1
]}

机构：

[1] Ist Italiano Tecnol, Ctr Biomol Nanotechnol UniLe, I-73010 Lecce, Italy

[2] Ist Nazl Tumori, Fdn IRCCS, Dept Expt Oncol & Mol Med, I-20133 Milan, Italy

[3] Fdn Ist FIRC Oncol Mol IFOM, Milan, Italy

[4] Ist Italiano Tecnol, I-16136 Genoa, Italy

来源：

ACS NANO | 2013年 / 7卷 / 06期

关键词：

biosensors; colorimetric; genotyping; gold nanoparticles; stacking Interactions; SINGLE-NUCLEOTIDE POLYMORPHISMS; KRAS MUTATION; GENOMIC DNA; ULTRASENSITIVE DETECTION; CHAIN-REACTION; NUCLEIC-ACID; AGGREGATION; OLIGONUCLEOTIDES; IDENTIFICATION; LIGASE;

D O I：

10.1021/nn401757w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Point mutations in the Kirsten rat sarcoma viral oncogene homologue (KRAS) gene are being increasingly recognized as Important diagnostic and prognostic markers in cancer. In this work, we describe a rapid and low-cost method for the naked-eye detection of cancer-related point mutations In KRAS based on gold nanoparticles. This simple colorimetric assay is sensitive (limit of detection in the low picomolar range), Instrument-free, and employs nonstringent room temperature conditions due to a combination of DNA-conjugated gold nanoparticles, a probe design which exploits cooperative hybridization for increased binding affinity, and signal enhancement on the surface of magnetic beads. Additionally, the scheme Is suitable for point-of-care applications, as it combines naked-eye detection, small sample volumes, and isothermal (PCR-free) amplification.

引用

页码：5530 / 5538

页数：9

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