Detection and phylogenetic analysis of novel crenarchaeote and euryarchaeote 16S ribosomal RNA gene sequences from a Great Barrier Reef sponge

被引：70

作者：

Webster, NS

Watts, JEM

Hill, RT

机构：

[1] Australian Inst Marine Sci, Townsville, Qld 4810, Australia

[2] Univ Maryland, Ctr Marine Biotechnol, Inst Biotechnol, Baltimore, MD 21202 USA

[3] James Cook Univ N Queensland, Dept Microbiol, Townsville, Qld 4814, Australia

来源：

MARINE BIOTECHNOLOGY | 2001年 / 3卷 / 06期

关键词：

marine; sponge; Archaea; 16S rRNA; crenarchaeote; euryarchaeote;

D O I：

10.1007/s10126-001-0065-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The presence of Archaea in the Great Barrier Reef marine sponge Rhopaloeides odorabile was investigated by 16S ribosomal RNA community analysis of total DNA extracted from the sponge tissue. The 16S rRNA gene sequences corresponding to group I crenarchaeotes and group II euryarchaeotes were recovered from R. odorabile tissue. The location of archaeal cells within the sponge tissue was investigated using fluorescently labeled oligonucleotide probes. The presence of Archaea was confirmed within all regions of the sponge tissue from R. odorabile, with a significantly higher number of archaeal cells located in the pinacoderm than the mesohyl region. This is the first report of euryarchaeaotes associated with marine sponges.

引用

页码：600 / 608

页数：9

共 41 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].