An Assay Suitable for High Throughput Screening of Anti-Influenza Drugs

被引：7

作者：

Mao, Lili ^{[1
,2
]}

Wang, Jun ^{[3
,4
]}

DeGrado, William F. ^{[3
,4
]}

Inouye, Masayori ^{[1
,2
]}

机构：

[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Biochem, Piscataway, NJ 08854 USA

[2] Robert Wood Johnson Med Sch, Ctr Adv Biotechnol & Med, Piscataway, NJ USA

[3] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA USA

[4] Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94143 USA

来源：

PLOS ONE | 2013年 / 8卷 / 01期

基金：

美国国家卫生研究院;

关键词：

INFLUENZA-A VIRUS; ION-CHANNEL; THERAPY; PROTEIN; PORE;

D O I：

10.1371/journal.pone.0054070

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.

引用

页数：5

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