Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection

被引：107

作者：

Jiang, Shanshan ^{[1
]}

Zhang, Yifan ^{[1
]}

Yang, Yichen ^{[1
]}

Huang, Yan ^{[1
]}

Ma, Gongcheng ^{[1
]}

Luo, Yongxiang ^{[1
]}

Huang, Peng ^{[1
]}

Lin, Jing ^{[1
]}

机构：

[1] Shenzhen Univ, Guangdong Key Lab Biomed Measurements & Ultrasoun, Lab Evolutionary Theranost, Int Canc Ctr,Sch Biomed Engn,Hlth Sci Ctr, Shenzhen 518060, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2019年 / 11卷 / 11期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

intracellular glucose; silver nanocube; glucose oxidase; fluorescence probe; signal amplification; HYDROGEN-PEROXIDE; SENSITIVE DETECTION; SILVER NANOPARTICLES; GOLD NANOPARTICLES; QUANTUM DOTS; CELL-GROWTH; PROBE; NANOCLUSTERS; GRAPHENE; DNA;

D O I：

10.1021/acsami.9b00010

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The accurate detection of glucose at cellular level remains a big challenge. In this study, a signal amplification strategy mediated by silver nanocube (AgNC), glucose oxidase (GOx), and silver ion fluorescence probe (denoted as AgNC Gox/Ag+-FP) is proposed for amplified intracellular glucose detection. The AgNC is oxidized into Ag+ by H2O2 generated from GOx-catalyzed glucose oxidation reaction, and Ag+ remarkably enhances the red fluorescence of Ag+-FP. Our results show that AgNC Gox/Ag+-FP is highly sensitive and specific to glucose and H2O2. Afterward, the feasibility of using AgNC Gox/Ag+-FP to detect intracellular glucose is verified in five different cell lines. In summary, a sensitive and specific fluorescence amplification strategy has been developed for intracellular glucose detection.

引用

页码：10554 / 10558

页数：5

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