MOLECULAR ANALYSIS OF THE COMPLETE GENOME SEQUENCE OF A TOBACCO ETCH VIRUS ISOLATE FROM CHINA

被引：1
作者：
Dai, J. [1 ,2 ]
Chen, W. [1 ,2 ]
Cheng, J. [3 ]
Wu, Y. [1 ,2 ]
机构：
[1] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Coll Plant Protect, Yangling 712100, Peoples R China
[2] Northwest A&F Univ, Key Lab Crop Pest Integrated Pest Management Loes, Minist Agr, Coll Plant Protect, Yangling 712100, Peoples R China
[3] Shaanxi Tobacco Res Inst, Xian 710061, Peoples R China
来源：
JOURNAL OF PLANT PATHOLOGY | 2015年 / 97卷 / 02期
关键词：
Potyvirus; genome; Tobacco etch virus; sequence analysis; HELPER COMPONENT-PROTEINASE; TURNIP-MOSAIC-VIRUS; PLUM-POX-VIRUS; APHID TRANSMISSIBILITY; NUCLEOTIDE-SEQUENCE; FAMILY POTYVIRIDAE; GENETIC-STRUCTURE; TERMINAL REGION; POINT MUTATION; CLEAVAGE SITE;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
An isolate of Tobacco etch virus (TEV-Shaanxi) induces systemic mosaic and necrotic lines or etching in N. Tabacum and is transmitted by M. persicae more efficiently than A. gossypii. The complete genome of TEV-Shaanxi consists of 9,494 nucleotides, excluding the 3' poly (A) tail. It contains a single large open reading frame (ORF) coding for a polyprotein of 3,054 amino acids, with an AUG start codon and a UGA stop codon. The polyprotein encodes 10 proteins (P1, HC-Pro, P3, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP), whose putative cleavage sites were identified by comparison with sequences of other known potyviruses. The identities of the 10 proteins of TEV-Shaanxi with other members of the genus Potyvirus were 30.5-62.7% and 29.4-68.8% at the nucleotide and amino acid levels, respectively. A short internal open reading frame, PIPO, was also highly conserved. TEV isolates whose sequences are available cluster into two groups which correspond with their host of origin.
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页码：297 / 301
页数：5
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共 28 条
  • [1] Overview and analysis of the polyprotein cleavage sites in the family Potyviridae
    Adams, MJ
    Antoniw, JF
    Beaudoin, F
    [J]. MOLECULAR PLANT PATHOLOGY, 2005, 6 (04) : 471 - 487
  • [2] Molecular criteria for genus and species discrimination within the family Potyviridae
    Adams, MJ
    Antoniw, JF
    Fauquet, CM
    [J]. ARCHIVES OF VIROLOGY, 2005, 150 (03) : 459 - 479
  • [3] A POINT MUTATION IN THE COAT PROTEIN ABOLISHES APHID TRANSMISSIBILITY OF A POTYVIRUS
    ATREYA, CD
    RACCAH, B
    PIRONE, TP
    [J]. VIROLOGY, 1990, 178 (01) : 161 - 165
  • [4] MUTATIONAL ANALYSIS OF THE HELPER COMPONENT-PROTEINASE GENE OF A POTYVIRUS - EFFECTS OF AMINO-ACID SUBSTITUTIONS, DELETIONS, AND GENE REPLACEMENT ON VIRULENCE AND APHID TRANSMISSIBILITY
    ATREYA, CD
    PIRONE, TP
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) : 11919 - 11923
  • [5] A VIRAL CLEAVAGE SITE CASSETTE - IDENTIFICATION OF AMINO-ACID SEQUENCES REQUIRED FOR TOBACCO ETCH VIRUS POLYPROTEIN PROCESSING
    CARRINGTON, JC
    DOUGHERTY, WG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) : 3391 - 3395
  • [6] NUCLEOTIDE-SEQUENCE OF A STRAIN OF TOBACCO ETCH VIRUS THAT DOES NOT CAUSE TABASCO PEPPER WILT
    CHU, MH
    JOHNSON, M
    THORNBURY, D
    BLACK, L
    PIRONE, T
    [J]. VIRUS GENES, 1995, 10 (03) : 283 - 288
  • [7] An overlapping essential gene in the Potyviridae
    Chung, Betty Y. -W.
    Miller, W. Allen
    Atkins, John F.
    Firth, Andrew E.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (15) : 5897 - 5902
  • [8] A multiplex reverse transcription PCR assay for simultaneous detection of five tobacco viruses in tobacco plants
    Dai, Jin
    Cheng, Julong
    Huang, Ting
    Zheng, Xuan
    Wu, Yunfeng
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2012, 183 (01) : 57 - 62
  • [9] The Determinant of Potyvirus Ability to Overcome the RTM Resistance of Arabidopsis thaliana Maps to the N-Terminal Region of the Coat Protein
    Decroocq, V.
    Salvador, B.
    Sicard, O.
    Glasa, M.
    Cosson, P.
    Svanella-Dumas, L.
    Revers, F.
    Garcia, J. A.
    Candresse, T.
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2009, 22 (10) : 1302 - 1311
  • [10] POSTTRANSLATIONAL PROCESSING OF THE TOBACCO ETCH VIRUS 49-KDA SMALL NUCLEAR INCLUSION POLYPROTEIN - IDENTIFICATION OF AN INTERNAL CLEAVAGE SITE AND DELIMITATION OF VPG AND PROTEINASE DOMAINS
    DOUGHERTY, WG
    PARKS, TD
    [J]. VIROLOGY, 1991, 183 (02) : 449 - 456
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