Maintenance of cellular ATP level by caloric restriction correlates chronological survival of budding yeast

被引:21
作者
Choi, Joon-Seok [1 ]
Lee, Cheol-Koo [1 ]
机构
[1] Korea Univ, Div Biotechnol, Coll Life Sci & Biotechnol, Seoul 136701, South Korea
基金
新加坡国家研究基金会;
关键词
Caloric restriction; Chronological lifespan; Aging; ATP; Budding yeast; OUTER MITOCHONDRIAL COMPARTMENT; FREE-RADICAL THEORY; LIFE-SPAN; SACCHAROMYCES-CEREVISIAE; SUPEROXIDE-DISMUTASE; STATIONARY-PHASE; OXIDATIVE DAMAGE; REDOX STATE; PROTEIN; EXTENSION;
D O I
10.1016/j.bbrc.2013.08.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The free radical theory of aging emphasizes cumulative oxidative damage in the genome and intracellular proteins due to reactive oxygen species (ROS), which is a major cause for aging. Caloric restriction (CR) has been known as a representative treatment that prevents aging; however, its mechanism of action remains elusive. Here, we show that CR extends the chronological lifespan (CLS) of budding yeast by maintaining cellular energy levels. CR reduced the generation of total ROS and mitochondrial superoxide; however, CR did not reduce the oxidative damage in proteins and DNA. Subsequently, calorie-restricted yeast had higher mitochondrial membrane potential (MMP), and it sustained consistent ATP levels during the process of chronological aging. Our results suggest that CR extends the survival of the chronologically aged cells by improving the efficiency of energy metabolism for the maintenance of the ATP level rather than reducing the global oxidative damage of proteins and DNA. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:126 / 131
页数:6
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