Biophysical characterization of the interaction between human serum albumin and n-dodecyl β-D-maltoside: A multi-technique approach
被引:21
作者:
Ali, Mohd. Sajid
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King Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi ArabiaKing Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi Arabia
Ali, Mohd. Sajid
[1
]
Al-Lohedan, Hamad A.
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King Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi ArabiaKing Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi Arabia
Al-Lohedan, Hamad A.
[1
]
机构:
[1] King Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi Arabia
We have studied the effect of biocompatible sugar based surfactant n-dodecyl beta-D-maltoside (DDM) on the conformation of human serum albumin (HSA). A multi-technique approach was applied in order to understand the type of interaction and effect of DDM on the secondary and tertiary structure of HSA. Surface tension measurement showed that HSA shifted the critical micelle concentration (cmc) of the surfactant to the higher side that clarifies the complex formation between DDM and HSA which was also confirmed by UV absorption spectroscopy. Fluorescence quenching measurements showed that fluorescence of HSA was quenched by the addition of DDM with a prominent blue shift indicative of the involvement of hydrophobic interaction which was further confirmed by extrinsic fluorescence of organic dye 1-anilino-8-naphthalenesulfonate. Synchronous fluorescence measurement trends suggested that the hydrophobicity increases near the tryptophan residue while an increase in the polarity was observed near tyrosine residues. A collective information obtained by circular dicroism (CD) and Fourier-transform infra-red (FUR) spectroscopies along with dynamic light scattering revealed the partial unfolding of the protein. (C) 2015 Elsevier B.V. All rights reserved.
机构:
King Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi ArabiaKing Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi Arabia
Ali, Mohd Sajid
;
Al-Lohedan, Hamad A.
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King Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi ArabiaKing Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi Arabia
机构:
Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
Armstrong, JK
;
Wenby, RB
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Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
Wenby, RB
;
Meiselman, HJ
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Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
Meiselman, HJ
;
Fisher, TC
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Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Botelho, MG
;
Gralle, M
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机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Gralle, M
;
Oliveira, CLP
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机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Oliveira, CLP
;
Torriani, I
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机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Torriani, I
;
Ferreira, ST
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机构:
Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
机构:
King Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi ArabiaKing Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi Arabia
Ali, Mohd Sajid
;
Al-Lohedan, Hamad A.
论文数: 0引用数: 0
h-index: 0
机构:
King Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi ArabiaKing Saud Univ, Dept Chem, Surfactant Res Chair, Riyadh 11451, Saudi Arabia
机构:
Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
Armstrong, JK
;
Wenby, RB
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
Wenby, RB
;
Meiselman, HJ
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
Meiselman, HJ
;
Fisher, TC
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USAUniv So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA
机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Botelho, MG
;
Gralle, M
论文数: 0引用数: 0
h-index: 0
机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Gralle, M
;
Oliveira, CLP
论文数: 0引用数: 0
h-index: 0
机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Oliveira, CLP
;
Torriani, I
论文数: 0引用数: 0
h-index: 0
机构:Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil
Torriani, I
;
Ferreira, ST
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Dept Med Biochem, BR-21944590 Rio De Janeiro, Brazil