LY294002 inhibits the Warburg effect in gastric cancer cells by downregulating pyruvate kinase M2

被引：32
作者：
Lu, Jian [1 ,2 ,3 ]
Chen, Min [1 ,3 ]
Gao, Sumeng [3 ]
Yuan, Jigang [3 ]
Zhu, Zhu [3 ]
Zou, Xiaoping [1 ,3 ]
机构：
[1] Nanjing Med Univ, Dept Gastroenterol, Affiliated Drum Tower Clin Med Sch, 321 Zhongshan Rd, Nanjing 210008, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Gastroenterol, Affiliated Wuxi Hosp 2, Wuxi 214002, Jiangsu, Peoples R China
[3] Nanjing Univ, Dept Gastroenterol, Affiliated Dram Tower Hosp, Sch Med, Nanjing 210008, Jiangsu, Peoples R China
来源：
ONCOLOGY LETTERS | 2018年 / 15卷 / 04期
基金：
美国国家科学基金会;
关键词：
LY294002; gastric cancer; Warburg effect; pyruvate kinase M2; glycolysis; PI3K/AKT/MTOR PATHWAY; AEROBIC GLYCOLYSIS; GLUCOSE-METABOLISM; UP-REGULATION; PKM2; EXPRESSION; PROLIFERATION; ISOFORM; METASTASIS; KNOCKDOWN;
D O I：
10.3892/ol.2018.7843
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The 'Warburg effect' is considered a vital hallmark of cancer cells, characterized by an altered metabolism, in which cells rely on aerobic glycolysis. As a key enzyme of aerobic glycolysis, pyruvate kinase M2 (PKM2) serves a crucial role in tumorigenesis. Accumulating studies have indicated that PKM2 is a potential target for cancer therapy. The aim of the present study was to assess the anticancer effects of LY294002, a specific phosphatidylinositol-3-kinase inhibitor, on gastric cancer (GC) cells and further explore its possible mechanism in vitro. The present study revealed that LY294002 inhibited GC cell proliferation, induced early apoptosis and significantly decreased lactate dehydrogenase activity and lactate production, in part through inhibiting PKM2 expression. In summary, LY294002 exhibits anticancer effects on GC, partly via the downregulation of PKM2.
引用
收藏
页码：4358 / 4364
页数：7
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