A highly selective turn-on fluorescent sensor for Cu(II) based on an NSe2 chelating moiety and its application in living cell imaging

被引：83

作者：

Chou, Cho-Yen ^{[1
]}

Liu, Shi-Rong ^{[1
]}

Wu, Shu-Pao ^{[1
]}

机构：

[1] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 300, Taiwan

来源：

ANALYST | 2013年 / 138卷 / 11期

关键词：

BODIPY DYES; CARBON DOTS; CU2+; COPPER; ION; WATER; RECOGNITION; CHEMOSENSOR;

D O I：

10.1039/c3an00286a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, a boron-dipyrromethene (BODIPY)-based fluorescent chemosensor CBS was developed for metal ion sensing. It was found that CBS containing an NSe2 moiety exhibited high selectivity for Cu2+ detection while CBS in the presence of Cu2+ displayed significant fluorescence enhancement. However, the metal ions Ag+, Ca2+, Co2+, Cr3+, Fe2+, Fe3+, Hg2+, K+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+ produced only minor changes in the fluorescence values of the system. The binding constant (K-a) of Cu2+ binding to CBS was found to be 7.28 x 10(3) M-1. The binding ratio of CBS-Cu2+ complexes was determined from the Job plot to be 1 : 1. The maximum fluorescence enhancement caused by Cu2+ binding to CBS was observed over the pH range 5.0-9.0. Additionally, the methyl thiazolyl tetrazolium (MTT) assay demonstrated the CBS to have low cytotoxicity. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that CBS could be used as an effective fluorescent probe for detecting Cu2+ in living cells.

引用

页码：3264 / 3270

页数：7

共 37 条

[1] Triple channel responsive Cu2+ probe [J].

Algi, Melek Pamuk ;

Oztas, Zahide ;

Algi, Fatih .

CHEMICAL COMMUNICATIONS, 2012, 48 (82) :10219-10221

[2] Neurodegenerative diseases and oxidative stress [J].

Barnham, KJ ;

Masters, CL ;

Bush, AI .

NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (03) :205-214

[3] Quantitative imaging of selenium, copper, and zinc in thin sections of biological tissues (Slugs-Genus arion) measured by laser ablation inductively coupled of plasma mass spectrometry [J].

Becker, J. S. ;

Matusch, A. ;

Depboylu, C. ;

Dobrowolska, J. ;

Zoriy, M. V. .

ANALYTICAL CHEMISTRY, 2007, 79 (16) :6074-6080

[4] A SPECTROPHOTOMETRIC INVESTIGATION OF THE INTERACTION OF IODINE WITH AROMATIC HYDROCARBONS [J].

BENESI, HA ;

HILDEBRAND, JH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1949, 71 (08) :2703-2707

[5] 4,4′-Difluoro-4-bora-3a,4a-diaza-s-indacenes (BODIPYs) as components of novel light active materials [J].

Benstead, Michael ;

Mehl, Georg H. ;

Boyle, Ross W. .

TETRAHEDRON, 2011, 67 (20) :3573-3601

[6] Copper and prion disease [J].

Brown, DR .

BRAIN RESEARCH BULLETIN, 2001, 55 (02) :165-173

[7] EFFECT OF SOLVENT POLARITY ON NONRADIATIVE PROCESSES IN XANTHENE DYES - RHODAMINE-B IN NORMAL ALCOHOLS [J].

CASEY, KG ;

QUITEVIS, EL .

JOURNAL OF PHYSICAL CHEMISTRY, 1988, 92 (23) :6590-6594

[8]

Cowan J.A., 1997, INORGANIC BIOCH INTR, P133

[9] Development of an oxidative dehydrogenation-based fluorescent probe for Cu2+ and its biological imaging in living cells [J].

Fan, Jiangli ;

Liu, Xiaojian ;

Hu, Mingming ;

Zhu, Hao ;

Song, Fengling ;

Peng, Xiaojun .

ANALYTICA CHIMICA ACTA, 2012, 735 :107-113

[10] Peat as a natural solid-phase for copper preconcentration and determination in a multicommuted flow system coupled to flame atomic absorption spectrometry [J].

Gonzales, A. P. S. ;

Firmino, M. A. ;

Nomura, C. S. ;

Rocha, F. R. P. ;

Oliveira, P. V. ;

Gaubeur, I. .

ANALYTICA CHIMICA ACTA, 2009, 636 (02) :198-204

← 1 2 3 4 →