Chondrogenic Differentiation in vitro of Murine Two-Factor Induced Pluripotent Stem Cells is Comparable to Murine Embryonic Stem Cells

被引：4
作者：
Kuboth, Sina [1 ]
Kramer, Jan [1 ]
Rohwedel, Juergen [1 ]
机构：
[1] Med Univ Lubeck, Dept Virol & Cell Biol, DE-23538 Lubeck, Germany
来源：
CELLS TISSUES ORGANS | 2012年 / 196卷 / 06期
关键词：
Chondrogenesis; In vitro differentiation; Murine induced pluripotent stem cells; Murine embryonic stem cells; FIBROBLASTS; LINES; CARDIOMYOCYTES; CHONDROCYTES; ACTIVATION; EXPRESSION; GENERATION; LINEAGES; INVITRO; MEMORY;
D O I：
10.1159/000338527
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Differentiation of embryonic stem (ES) cells via embryoid bodies has been established as an appropriate model to study the development of various cell types in vitro. Here, we show that murine induced pluripotent stem (iPS) cells, reprogrammed by exogenous expression of the two transcription factors Oct4 and Klf4 (2F OK iPS), differentiate into chondrocytes in vitro characterized by the appearance of Alcian blue-stained nodules and the expression of cartilage-associated genes and proteins. Quantitatively, the chondrogenic differentiation potential of 2F OK iPS and ES cells was found to be similar. Further, we demonstrate the induction of chondrogenic iPS cell differentiation by certain members of the transforming growth factor-beta family (BMP-2, TGF-beta(1)). The number of Alcian blue-positive nodules and the expression of the cartilage marker molecule collagen type II increased after application of BMP-2, whereas simultaneous treatment with both BMP-2 and TGF-beta(1) showed no significant effect on gene expression. Copyright (C) 2012 S. Karger AG, Basel
引用
收藏
页码：481 / 489
页数：9
相关论文
共 31 条
[1]  
DOETSCHMAN TC, 1985, J EMBRYOL EXP MORPH, V87, P27
[2]   ESTABLISHMENT IN CULTURE OF PLURIPOTENTIAL CELLS FROM MOUSE EMBRYOS [J].
EVANS, MJ ;
KAUFMAN, MH .
NATURE, 1981, 292 (5819) :154-156
[3]   Human embryonic stem cells: emerging technologies and practical applications [J].
Fenno, Lief E. ;
Ptaszek, Leon M. ;
Cowan, Chad A. .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2008, 18 (04) :324-329
[4]   Notch1 signaling regulates chondrogenic lineage determination through Sox9 activation [J].
Haller, R. ;
Schwanbeck, R. ;
Martini, S. ;
Bernoth, K. ;
Kramer, J. ;
Just, U. ;
Rohwedel, J. .
CELL DEATH AND DIFFERENTIATION, 2012, 19 (03) :461-469
[5]   Loss of Sox9 function results in defective chondrocyte differentiation of mouse embryonic stem cells in vitro [J].
Hargus, Gunnar ;
Kist, Ralf ;
Kramer, Jan ;
Gerstel, Daniela ;
Neitz, Angela ;
Scherer, Gerd ;
Rohwedel, Juergen .
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2008, 52 (04) :323-332
[6]   Differentiation plasticity of chondrocytes derived from mouse embryonic stem cells [J].
Hegert, C ;
Kramer, J ;
Hargus, G ;
Müller, J ;
Guan, K ;
Wobus, AM ;
Müller, PK ;
Rohwedel, J .
JOURNAL OF CELL SCIENCE, 2002, 115 (23) :4617-4628
[7]   Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency [J].
Hu, Bao-Yang ;
Weick, Jason P. ;
Yu, Junying ;
Ma, Li-Xiang ;
Zhang, Xiao-Qing ;
Thomson, James A. ;
Zhang, Su-Chun .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (09) :4335-4340
[8]   Cell line-dependent differentiation of induced pluripotent stem cells into cardiomyocytes in mice [J].
Kaichi, Shinji ;
Hasegawa, Koji ;
Takaya, Tomohide ;
Yokoo, Noritaka ;
Mima, Takahiro ;
Kawamura, Teruhisa ;
Morimoto, Tatsuya ;
Ono, Koh ;
Baba, Shiro ;
Doi, Hiraku ;
Yamanaka, Shinya ;
Nakahata, Tatsutoshi ;
Heike, Toshio .
CARDIOVASCULAR RESEARCH, 2010, 88 (02) :314-323
[9]   iPS Cells Can Support Full-Term Development of Tetraploid Blastocyst-Complemented Embryos [J].
Kang, Lan ;
Wang, Jianle ;
Zhang, Yu ;
Kou, Zhaohui ;
Gao, Shaorong .
CELL STEM CELL, 2009, 5 (02) :135-138
[10]   Differences between Cellular and Molecular Profiles of Induced Pluripotent Stem Cells Generated from Mouse Embryonic Fibroblasts [J].
Kim, Eun Young ;
Jeon, Kilsoo ;
Park, Hyo Young ;
Han, Young Joon ;
Yang, Byoung Chul ;
Park, Soo Bong ;
Chung, Hyung Min ;
Park, Se Pill .
CELLULAR REPROGRAMMING, 2010, 12 (06) :627-639
1234