The use of rpoB sequence analysis in the differentiation of Mycobacterium abscessus and Mycobacterium chelonae: a critical judgement in cystic fibrosis?

被引:9
作者
Arnold, C. [1 ]
Barrett, A. [2 ]
Cross, L. [1 ]
Magee, J. G. [2 ]
机构
[1] Ctr Infect, HPA Microbiol Serv, Appl & Funct Genom Unit, London, England
[2] HPA Microbiol Serv, N England Mycobacterium Reference Lab, Newcastle Upon Tyne, Tyne & Wear, England
关键词
M; chelonae; abscessus; cystic fibrosis; sequencing; rpoB; SP-NOV; IDENTIFICATION; MASSILIENSE; GENE; INFECTION; BOLLETII; PATIENT; SPUTUM;
D O I
10.1111/j.1469-0691.2012.03785.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Individuals suffering from fibrocystic disease may acquire non-tuberculous mycobacteria as colonizing or infecting organisms. Mycobacterium abscessus is of particular concern because it may be very difficult to eradicate and may mitigate against lung transplantation. However, this species may be difficult to reliably differentiate from the closely related M. chelonae. We have developed a rapid, low-cost, short sequence-based technique to confirm species identity by analysis of a segment of the RNA Polymerase B (rpoB) gene.
引用
收藏
页码:E131 / E133
页数:3
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