共 49 条
BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways
被引:88
作者:
Passarinho, Paul
[1
]
Ketelaar, Tijs
[2
]
Xing, Meiqing
[1
]
van Arkel, Jeroen
[1
]
Maliepaard, Chris
[3
]
Hendriks, Mieke Weemen
[1
]
Joosen, Ronny
[1
]
Lammers, Michiel
[1
]
Herdies, Lydia
[4
]
den Boer, Bart
[4
]
van der Geest, Lonneke
[1
]
Boutilier, Kim
[1
]
机构:
[1] Plant Res Int, NL-6700 AA Wageningen, Netherlands
[2] Wageningen Univ, Lab Plant Cell Biol, NL-6700 AJ Wageningen, Netherlands
[3] Wageningen Univ, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands
[4] Bayer BioSci NV, B-9052 Ghent, Belgium
关键词:
AP2;
ERF transcription factor;
BABY BOOM;
Arabidopsis;
somatic embryo;
actin;
D O I:
10.1007/s11103-008-9364-y
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth.
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页码:225 / 237
页数:13
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