BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

被引:88
作者
Passarinho, Paul [1 ]
Ketelaar, Tijs [2 ]
Xing, Meiqing [1 ]
van Arkel, Jeroen [1 ]
Maliepaard, Chris [3 ]
Hendriks, Mieke Weemen [1 ]
Joosen, Ronny [1 ]
Lammers, Michiel [1 ]
Herdies, Lydia [4 ]
den Boer, Bart [4 ]
van der Geest, Lonneke [1 ]
Boutilier, Kim [1 ]
机构
[1] Plant Res Int, NL-6700 AA Wageningen, Netherlands
[2] Wageningen Univ, Lab Plant Cell Biol, NL-6700 AJ Wageningen, Netherlands
[3] Wageningen Univ, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands
[4] Bayer BioSci NV, B-9052 Ghent, Belgium
关键词
AP2; ERF transcription factor; BABY BOOM; Arabidopsis; somatic embryo; actin;
D O I
10.1007/s11103-008-9364-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth.
引用
收藏
页码:225 / 237
页数:13
相关论文
共 49 条
[1]   The PLETHORA genes mediate patterning of the Arabidopsis root stem cell niche [J].
Aida, M ;
Beis, D ;
Heidstra, R ;
Willemsen, V ;
Blilou, I ;
Galinha, C ;
Nussaume, L ;
Noh, YS ;
Amasino, R ;
Scheres, B .
CELL, 2004, 119 (01) :109-+
[2]   Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues [J].
An, YQ ;
McDowell, JM ;
Huang, SR ;
McKinney, EC ;
Chambliss, S ;
Meagher, RB .
PLANT JOURNAL, 1996, 10 (01) :107-121
[3]   Overexpression of Arabidopsis ESR1 induces initiation of shoot regeneration [J].
Banno, H ;
Ikeda, Y ;
Niu, QW ;
Chua, NH .
PLANT CELL, 2001, 13 (12) :2609-2618
[4]   Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth [J].
Boutilier, K ;
Offringa, R ;
Sharma, VK ;
Kieft, H ;
Ouellet, T ;
Zhang, LM ;
Hattori, J ;
Liu, CM ;
van Lammeren, AAM ;
Miki, BLA ;
Custers, JBM ;
Campagne, MMV .
PLANT CELL, 2002, 14 (08) :1737-1749
[5]   Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis [J].
Braybrook, SA ;
Stone, SL ;
Park, S ;
Bui, AQ ;
Le, BH ;
Fischer, RL ;
Goldberg, RB ;
Harada, JJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (09) :3468-3473
[6]   Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: Implication in actin-based motility [J].
Carlier, MF ;
Laurent, V ;
Santolini, J ;
Melki, R ;
Didry, D ;
Xia, GX ;
Hong, Y ;
Chua, NH ;
Pantaloni, D .
JOURNAL OF CELL BIOLOGY, 1997, 136 (06) :1307-1322
[7]   Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis [J].
Casson, S ;
Spencer, M ;
Walker, K ;
Lindsey, K .
PLANT JOURNAL, 2005, 42 (01) :111-123
[8]   Developmental steps in acquiring competence for shoot development in Arabidopsis tissue culture [J].
Che, Ping ;
Lall, Sonia ;
Howell, Stephen H. .
PLANTA, 2007, 226 (05) :1183-1194
[9]   Gene expression programs during shoot, root, and callus development in Arabidopsis tissue culture [J].
Che, Ping ;
Lall, Sonia ;
Nettleton, Dan ;
Howell, Stephen H. .
PLANT PHYSIOLOGY, 2006, 141 (02) :620-637
[10]   KNAT1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis [J].
Chuck, G ;
Lincoln, C ;
Hake, S .
PLANT CELL, 1996, 8 (08) :1277-1289