Applicability of FTIR-ATR Method to Measure Carbonyls in Blood Plasma after Physical and Mental Stress

被引:29
作者
Bujok, Jolanta [1 ]
Gasior-Glogowska, Marlena [2 ]
Marszalek, Michal [3 ]
Trochanowska-Pauk, Natalia [2 ]
Zigo, Frantisek [4 ]
Pavl'ak, Alexander [4 ]
Komorowska, Malgorzata [2 ]
Walski, Tomasz [2 ,5 ]
机构
[1] Wroclaw Univ Environm & Life Sci, Fac Vet Med, Dept Anim Physiol & Biostruct, Norwida 31, PL-50375 Wroclaw, Poland
[2] Wroclaw Univ Sci & Technol, Fac Fundamental Problems Technol, Dept Biomed Engn, Wybrzeze Wyspianskiego 27, PL-50375 Wroclaw, Poland
[3] Wroclaw Univ Sci & Technol, Fac Chem, Wybrzeze Wyspianskiego 27, PL-50370 Wroclaw, Poland
[4] Univ Vet Med & Pharm Kosice, Dept Anim Husb, Komenskeho 73, Kosice 04181, Slovakia
[5] Natl Univ Ireland Galway, Ctr Res Med Devices CURAM, Biosci Res Bldg 118, Galway, Ireland
基金
爱尔兰科学基金会; 欧盟地平线“2020”;
关键词
INFRARED-SPECTROSCOPY; IDENTIFICATION; PROTEINS; SERUM;
D O I
10.1155/2019/2181370
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Introduction. Oxidative stress is a state of imbalance between the production of reactive oxygen species and antioxidant defenses. It results in the oxidation of all cellular elements and, to a large extent, proteins, causing inter alia the formation of carbonyl groups in their structures. The study focused on assessment of changes in the plasma protein-bound carbonyls in police horses after combat training and after rest and the applicability of infrared spectroscopy with a Fourier transform, utilizing the attenuated total reflectance (FTIR-ATR) in detecting plasma protein oxidation. Methods. We evaluated the influence of both the different concentrations of hydrogen peroxide and combat training on protein carbonylation in horse blood plasma. The oxidation of plasma proteins was assessed using a spectrophotometric method based on the carbonyl groups derivatization with 2,4-dinitrophenylhydrazine (DNPH). The measured values were correlated with the carbonyl groups concentrations determined by means of the FTIR-ATR method. Results. The linear correlation between the DNPH and FTIR-ATR methods was shown. The concentration of plasma protein-bound carbonyls significantly deceased in police horses after one-day rest when compared to the values measured directly after the combat training (a drop by 23%, p<0.05 and 29%, p<0.01 measured by DNPH and FTIR-ATR methods, respectively). These results were consistent with the proteins phosphorylation analysis. Conclusion. The FTIR-ATR method may be applied to measure the level of plasma proteins peroxidation.
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页数:9
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