Antigen-presenting property of mesenchymal stem cells occurs during a narrow window at low levels of interferon-γ

Mesenchymal stem cells (MSCs) are mostly found around the vasculature system of the adult bone marrow (BM). They function as immune suppressors, express MHC-II, are phagocytic, and support T-cell cytotoxicity. We hypothesize that these contradictory properties of MSCs are important for BM homeostasis and occur partly through antigen presentation (antigen-presenting cells [APCs]) within a narrow window. Indeed, we have verified APC functions of MScs to recall antigens, Candida albicans and Tetanus toxoid. The target cells have been identified to be CD4(+) T cells. APC assays with IFN gamma-knockdown MSCs and with anti-IFN gamma receptor confirmed that MHC-II expression requires autocrine stimulation by IFN gamma. During APC functions, as IFN gamma levels become elevated, there was a concomitant decrease in MHC-II on MSCs. This observation was correlated with flow cytometry studies showing a gradual decrease in MHC-II expression as IFN gamma levels were increased. The reduced levels of MHC-II correlated with losses in their allogeneic potential, as indicated in mixed lymphocyte reaction. In summary, endogenous and low levels of IFN gamma are required for MHC-II expression on MSCs, and for APC functions. APC functions occur during a narrow window before IFN-gamma levels are increased. The study has implications for BM protection against infection and exacerbated inflammatory responses.