Effect of Metal Ions on the Hydrolytic and Transesterification Activities of Candida rugosa Lipase

被引：17

作者：

Katiyar, Madhu ^{[1
]}

Ali, Amjad ^{[1
]}

机构：

[1] Thapar Univ, Sch Chem & Biochem, Patiala 147004, Punjab, India

来源：

JOURNAL OF OLEO SCIENCE | 2013年 / 62卷 / 11期

关键词：

kinetics; transesterification; biodiesel; Candida rugosa lipase; metal ions; activation energy; COTTON SEED OIL; BIODIESEL PRODUCTION; CATALYTIC-PROPERTIES; IMMOBILIZED LIPASE; VEGETABLE-OILS; CASTOR-OIL; SPECTROSCOPY;

D O I：

10.5650/jos.62.919

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In order to study the effect of metal ions on lipase activity, hydrolytic and transesterification activities of Candida rugosa lipase were investigated in presence of alkali (Na+ and K+), alkaline earth (Ca+2 and Ba+2) and transition (Cr+3, Fe+3, Co+2, Cu+2 and Ni+2) metal ions. Maximum enhancement in hydrolytic activity of lipase was observed by Ca+2, and in transesterification activity by Cr+3 and Co+2. The kinetics of the lipase catalyzed transesterification (methanolysis and ethanolysis) reactions were also studied, and the activation energies of methanolysis and ethanolysis were reduced from 10.16 and 10.24 kcal mol(-1), respectively, to 5.41 and 7.55 kcal mol(-1), respectively, when reactions were performed in presence of Co+2. Thus, in lipase catalyzed transesterification Cr+3 or Co+2 could be added to the assay in order to produce the biodiesel in relatively shorter reaction duration.

引用

页码：919 / 924

页数：6

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