Molecular Characterization, Expression Patterns, and Association Analysis with Carcass Traits of Porcine USF1 Gene

被引：14

作者：

Wu, Huayu ^{[1
]}

Qiao, Mu ^{[1
]}

Peng, Xianwen ^{[1
]}

Wu, Junjing ^{[1
]}

Liu, Guisheng ^{[1
]}

Sun, Hua ^{[1
]}

Li, Lianghua ^{[1
]}

Mei, Shuqi ^{[1
]}

机构：

[1] Hubei Acad Agr Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Inst Anim Husb & Vet, Wuhan 430064, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2013年 / 170卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Alternative splicing; Association analysis; Porcine; SNP; USF1; FAMILIAL COMBINED HYPERLIPIDEMIA; UPSTREAM TRANSCRIPTION FACTOR-1; LOCI; CHROMOSOME-4; FATNESS; GROWTH; PIGS; POLYMORPHISM; PROTEINS; DISEASE;

D O I：

10.1007/s12010-013-0280-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The upstream stimulatory factor 1 (USF1) is a transcription factor controlling expression of several genes involved in lipid and glucose homeostasis. In this study, two isoforms of the porcine USF1 gene were detected by reverse transcription polymerase chain reaction (RT-PCR), termed USF1 wild-type (wt) and USF1/CD, both of them contain a helix-loop-helix leucine zipper (HLH-LZ) conserved domain. Tissue distribution analysis showed that the two transcripts of porcine USF1 gene were ubiquitously expressed in all tested tissues, except for heart. Moreover, we found that a single nucleotide polymorphism (SNP, C/T) in intron 10 was significantly associated with ratio of lean to fat (P < 0.05), dress percentage (P < 0.05), average backfat thickness (P < 0.05), loin eye width (P < 0.05), lean meat percentage (P < 0.01), loin eye height (P < 0.01), and loin eye area (P < 0.01). This result suggests that porcine USF1 gene may be a candidate gene of meat production trait.

引用

页码：1310 / 1319

页数：10

共 27 条

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