ICAT participates in proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cell

被引：11
作者：
Kim, Yeon Jeong [1 ,2 ]
Kim, Jong Tae [1 ,2 ]
Bae, Yong Chan [3 ]
Suh, Kuen Taek [4 ]
Jung, Jin Sup [1 ,2 ,5 ]
机构：
[1] Pusan Natl Univ, Sch Med, Dept Physiol, Pusan 602739, South Korea
[2] Pusan Natl Univ, Med Res Ctr Ischem Tissue Engn, Pusan 602739, South Korea
[3] Pusan Natl Univ, Sch Med, Dept Plast Surg, Pusan 602739, South Korea
[4] Pusan Natl Univ, Sch Med, Dept Orthoped Surg, Pusan 602739, South Korea
[5] Pusan Natl Univ, Med Res Inst, Pusan 602739, South Korea
来源：
LIFE SCIENCES | 2008年 / 83卷 / 25-26期
关键词：
ICAT; Adipose tissue; Mesenchymal stem cells; Proliferation; Osteogenic differentiation;
D O I：
10.1016/j.lfs.2008.09.030
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Aims: The Wnt/beta-catenin pathway plays a critical part in several cell physiology events associated with embryonic development and adult homeostasis, including determination, proliferation, migration, and differentiation. However, the role of Wnt signaling in osteoblastogenesis from mesenchymal stem cells (MSC) remains a controversial matter. Therefore, in the present study, we investigated how ICAT (inhibitor of beta-catenin and TCF-4), a negative regulator of the Wnt signaling pathway, influenced differentiation and proliferation of human adipose tissue-derived stromal cells (hASC). Main methods: To mediate ICAT overexpression in hASC, we used a lentiviral gene transfer technique. We further determined the role of ICAT by RNAi technique. Key findings: ICAT-transduced hASC exhibited lower TCF promoter activity and Cellular growth capacity than control cells, but [CAT overexpression did not affect hASC attachment efficiency. ICAT overexpression also increased osteogenic differentiation. Conversely, introduction of an ICAT siRNA oligonucleotide increased TCF promoter activity and cellular proliferation, but it inhibited osteogenic differentiation. Significance: Taken together, these findings indicated that ICAT participated in regulating hASC proliferation and differentiation by modulating Wnt signaling. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：851 / 858
页数：8
相关论文
共 35 条
[1]   Wnt 3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cells [J].
Boland, GM ;
Perkins, G ;
Hall, DJ ;
Tuan, RS .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2004, 93 (06) :1210-1230
[2]   High bone density due to a mutation in LDL-receptor-related protein 5 [J].
Boyden, LM ;
Mao, JH ;
Belsky, J ;
Mitzner, L ;
Farhi, A ;
Mitnick, MA ;
Wu, DQ ;
Insogna, K ;
Lifton, RP .
NEW ENGLAND JOURNAL OF MEDICINE, 2002, 346 (20) :1513-1521
[3]   Endogenous Wnt signaling promotes proliferation and suppresses osteogenic differentiation in human adipose derived stromal cells [J].
Cho, HH ;
Kim, YJ ;
Kim, SJ ;
Kim, JH ;
Bae, YC ;
Ba, B ;
Jung, JS .
TISSUE ENGINEERING, 2006, 12 (01) :111-121
[4]   ICAT inhibits β-catenin binding to Tcf/Lef-family transcription factors and the general coactivator p300 using independent structural modules [J].
Daniels, DL ;
Weis, WI .
MOLECULAR CELL, 2002, 10 (03) :573-584
[5]   Wnt signaling inhibits osteogenic differentiation of human mesenchymal stem cells [J].
de Boer, J ;
Siddappa, R ;
Gaspar, C ;
van Apeldoorn, A ;
Fodde, R ;
van Blitterswijk, C .
BONE, 2004, 34 (05) :818-826
[6]   Differentiation potential of adipose derived adult stem (ADAS) cells [J].
Gimble, JM ;
Guilak, F .
CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY, VOL 58, 2003, 58 :137-160
[7]   LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development [J].
Gong, YQ ;
Slee, RB ;
Fukai, N ;
Rawadi, G ;
Roman-Roman, S ;
Reginato, AM ;
Wang, HW ;
Cundy, T ;
Glorieux, FH ;
Lev, D ;
Zacharin, M ;
Oexle, K ;
Marcelino, J ;
Suwairi, W ;
Heeger, S ;
Sabatakos, G ;
Apte, S ;
Adkins, WN ;
Allgrove, J ;
Arslan-Kirchner, M ;
Batch, JA ;
Beighton, P ;
Black, GCM ;
Boles, RG ;
Boon, LM ;
Borrone, C ;
Brunner, HG ;
Carle, GF ;
Dallapiccola, B ;
De Paepe, A ;
Floege, B ;
Halfhide, ML ;
Hall, B ;
Hennekam, RC ;
Hirose, T ;
Jans, A ;
Jüppner, H ;
Kim, CA ;
Keppler-Noreuil, K ;
Kohlschuetter, A ;
LaCombe, D ;
Lambert, M ;
Lemyre, E ;
Letteboer, T ;
Peltonen, L ;
Ramesar, RS ;
Romanengo, M ;
Somer, H ;
Steichen-Gersdorf, E ;
Steinmann, B .
CELL, 2001, 107 (04) :513-523
[8]   Role for ICAT in β-catenin-dependent nuclear signaling and cadherin functions [J].
Gottardi, CJ ;
Gumbiner, BM .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2004, 286 (04) :C747-C756
[9]   The crystal structure of the β-catenin/ICAT complex reveals the inhibitory mechanism of ICAT [J].
Graham, TA ;
Clements, WK ;
Kimelman, D ;
Xu, WQ .
MOLECULAR CELL, 2002, 10 (03) :563-571
[10]   Identification of c-MYC as a target of the APC pathway [J].
He, TC ;
Sparks, AB ;
Rago, C ;
Hermeking, H ;
Zawel, L ;
da Costa, LT ;
Morin, PJ ;
Vogelstein, B ;
Kinzler, KW .
SCIENCE, 1998, 281 (5382) :1509-1512
1234