Interstitial fibrosis in the heart: differences in extracellular matrix proteins and matrix metalloproteinases in end-stage dilated, ischaemic and valvular cardiomyopathy

被引:52
作者
Herpel, E
Pritsch, M
Koch, A
Dengler, TJ
Schirmacher, P
Schnabel, PA
机构
[1] Heidelberg Univ, Dept Pathol, D-69120 Heidelberg, Germany
[2] Heidelberg Univ, Dept Med Biometry, D-69120 Heidelberg, Germany
[3] Heidelberg Univ, Dept Cardiac Surg, D-69120 Heidelberg, Germany
[4] Heidelberg Univ, Dept Internal Med, D-69120 Heidelberg, Germany
关键词
dilated cardiomyopathy; extracellular matrix; remodelling; ischaemic cardiomyopathy; valvular cardiomyopathy;
D O I
10.1111/j.1365-2559.2006.02398.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Aims: To investigate whether or not there are differences in the distribution of extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs) in end-stage heart failure underlying different cardiomyopathies. Methods and results: Thirty-nine explanted human hearts were investigated: 15 with dilated cardiomyopathy (DCM), 17 with ischaemic cardiomyopathy (ICM) and seven with valvular cardiomyopathy (VCM). Transmural samples from four different sites were investigated. Frozen sections were processed for immunohistochemistry for collagens type I, III, IV, laminin and fibronectin, as well as MMP-1, -2 and -9. Volume densities were determined. All ECM components were expressed more frequently in DCM than in ICM. Comparing ICM with VCM, all proteins were found more frequently in VCM than in ICM except for type III collagen, which was significantly more frequent in ICM. Comparing DCM and VCM, VCM showed significantly higher volume densities for type III collagen and laminin. MMPs showed only slight variations between the cardiomyopathies. Conclusion: The distribution of ECM proteins differs between DCM, ICM and VCM, which suggests that they can be morphologically discriminated by interstitial fibrosis, especially by their expression of matrix proteins.
引用
收藏
页码:736 / 747
页数:12
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