Local mechanical properties of bladder cancer cells measured by AFM as a signature of metastatic potential

被引:40
作者
Abidine, Y. [1 ,2 ]
Laurent, V. M. [1 ,2 ]
Michel, R. [1 ,2 ]
Duperray, A. [3 ,4 ]
Verdier, C. [1 ,2 ]
机构
[1] Univ Grenoble Alpes, LIPHY, F-38000 Grenoble, France
[2] LIPHY, CNRS, F-38000 Grenoble, France
[3] IAB, INSERM, F-38000 Grenoble, France
[4] Univ Grenoble Alpes, IAB, F-38000 Grenoble, France
来源
EUROPEAN PHYSICAL JOURNAL PLUS | 2015年 / 130卷 / 10期
关键词
ATOMIC-FORCE MICROSCOPY; ACTIN CYTOSKELETON; LIVING CELLS; ADHESION; MYOSIN; MICRORHEOLOGY; CONTRACTILITY; LATRUNCULIN; ELASTICITY; ACTOMYOSIN;
D O I
10.1140/epjp/i2015-15202-6
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
The rheological properties of bladder cancer cells of different invasivities have been investigated using a microrheological technique well adapted in the range [1-300 Hz] of interest to understand local changes in the cytoskeleton microstructure, in particular actin fibres. Drugs disrupting actin and actomyosin functions were used to study the resistance of such cancer cells. Results on a variety of cell lines were fitted with a model revealing the importance of two parameters, the elastic shear plateau modulus G(N)(0) as well as the glassy transition frequency f(T). These parameters are good markers for invasiveness, with the notable exception of the cell periphery, which is stiffer for less invasive cells, and could be of importance in cancer metastasis.
引用
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页数:13
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共 52 条
  • [41] Probing the viscoelastic behavior of cultured airway smooth muscle cells with atomic force microscopy:: Stiffening induced by contractile agonist
    Smith, BA
    Tolloczko, B
    Martin, JG
    Grütter, P
    [J]. BIOPHYSICAL JOURNAL, 2005, 88 (04) : 2994 - 3007
  • [42] Rheological constitutive equation for a model of soft glassy materials
    Sollich, P
    [J]. PHYSICAL REVIEW E, 1998, 58 (01) : 738 - 759
  • [43] Rheological Behavior of living cells is timescale-dependent
    Stamenovic, Dimitrije
    Rosenblatt, Noah
    Montoya-Zavala, Martin
    Matthews, Benjamin D.
    Hu, Shaohua
    Suki, Bela
    Wang, Ning
    Ingber, Donald E.
    [J]. BIOPHYSICAL JOURNAL, 2007, 93 (08) : L39 - L41
  • [44] Biomechanics and biophysics of cancer cells
    Suresh, Subra
    [J]. ACTA BIOMATERIALIA, 2007, 3 (04) : 413 - 438
  • [45] The role of F-actin and myosin in epithelial cell rheology
    Van Citters, Kathleen M.
    Hoffman, Brenton D.
    Massiera, Gladys
    Crocker, John C.
    [J]. BIOPHYSICAL JOURNAL, 2006, 91 (10) : 3946 - 3956
  • [46] Wakatsuki T, 2001, J CELL SCI, V114, P1025
  • [47] Increased plasticity of the stiffness of melanoma cells correlates with their acquisition of metastatic properties
    Weder, Gilles
    Hendriks-Balk, Marielle C.
    Smajda, Rita
    Rimoldi, Donata
    Liley, Martha
    Heinzelmann, Harry
    Meister, Andre
    Mariotti, Agnese
    [J]. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2014, 10 (01) : 141 - 148
  • [48] Mechanics of living cells measured by laser tracking microrheology
    Yamada, S
    Wirtz, D
    Kuo, SC
    [J]. BIOPHYSICAL JOURNAL, 2000, 78 (04) : 1736 - 1747
  • [49] Regulation of the actin cytoskeleton in cancer cell migration and invasion
    Yamaguchi, Hideki
    Condeelis, John
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2007, 1773 (05): : 642 - 652
  • [50] Yarmola EG, 2000, J BIOL CHEM, V275, P28120