Thrombin inhibitor from the salivary gland of the camel tick Hyalomma dromedarii

被引：10

作者：

Ibrahim, Mahmoud A. ^{[1
]}

Masoud, Hassan M. M. ^{[1
]}

机构：

[1] Natl Res Ctr, Dept Mol Biol, 33 El Bohouth St,PO 12622, Giza, Egypt

来源：

EXPERIMENTAL AND APPLIED ACAROLOGY | 2018年 / 74卷 / 01期

关键词：

Camel tick; Hyalomma dromedarii; Salivary gland; Thrombin inhibitor; ORNITHODOROS-SAVIGNYI ACARI; LONE STAR TICK; BOOPHILUS-MICROPLUS; AMBLYOMMA-AMERICANUM; IXODES-SCAPULARIS; FACTOR-XA; ANTICOAGULANT; IDENTIFICATION; PROTEIN; ORIGIN;

D O I：

10.1007/s10493-017-0196-9

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Blood-sucking arthropods have different types of anticoagulants to allow the ingestion of a blood meal from their hosts. In this study, five anticoagulants prolonging the activated partial thromboplastin time were resolved from the salivary gland crude extract of the camel tick Hyalomma dromedarii by chromatography on diethylaminoethyl (DEAE)-cellulose column. They were designated P1, P2, P3, P4 and P5 according to their elution order. P5 was found to be a potent thrombin inhibitor and purified by ultrafiltration through two centrifugal concentrators of 50 and 30 kDa molecular weight cut-off (MWCO), respectively. The camel tick salivary gland thrombin inhibitor was purified 60.6 folds with a specific activity of 564 units/mg protein. It turned out to be homogenous on native-PAGE with molecular weight of 36 kDa as detected on 12% SDS-PAGE. It inhibits bovine thrombin competitively with K (i) value of 0.55 mu M. A task for the future will be the elucidation of this thrombin inhibitor structure to allow its application in thrombosis treatment.

引用

页码：85 / 97

页数：13

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