ETB receptors;
ETA receptors;
circadian rhythm;
kidney;
male and female;
rats;
DUCT-SPECIFIC KNOCKOUT;
PROTEIN PERIOD 1;
II HYPERTENSIVE-RATS;
BLOOD-PRESSURE;
COLLECTING DUCT;
ETB-RECEPTOR;
DEFICIENT RATS;
ALPHA-ENAC;
HIGH-SALT;
EXPRESSION;
D O I:
10.1152/ajprenal.00103.2016
中图分类号:
Q4 [生理学];
学科分类号:
071003 ;
摘要:
Recent studies suggested a direct link between circadian rhythms and regulation of sodium excretion. Endothelin-1 (ET-1) regulates sodium balance by promoting natriuresis through the endothelin B receptor (ETB) in response to increased salt in the diet, but the effect that the time of day has on this natriuretic response is not known. Therefore, this study was designed to test the hypothesis that ETB receptor activation contributes to the diurnal control of sodium excretion and that sex differences contribute to this control as well. Twelve-hour urine collections were used to measure sodium excretion. On day 3 of the experiment, a NaCl load (900 mu eq) was given by oral gavage either at Zeitgeber time [ZT] 0 (inactive period) or ZT12 (active period) to examine the natriuretic response to the acute salt load. Male and female ETB-deficient (ETB def) rats showed an impaired natriuretic response to a salt load at ZT0 compared with their respective transgenic controls (Tg cont). Male ETB def rats showed a delayed natriuretic response to a salt load given at ZT12 compared with male Tg cont, a contrast to the prompt response shown by female ETB def rats. Treatment with ABT-627, an ETA receptor antagonist, improved the natriuretic response seen within the first 12 h of a ZT0 salt load in both sexes. These findings demonstrate that diurnal excretion of an acute salt load 1) requires ET-1 and the ETB receptor, 2) is more evident in male vs. female rats, and 3) is opposed by the ETA receptor.
机构:
Royal Infirm Edinburgh NHS Trust, Dept Renal Med, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Dhaun, Neeraj
Moorhouse, Rebecca
论文数: 0引用数: 0
h-index: 0
机构:Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Moorhouse, Rebecca
MacIntyre, Iain M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
MacIntyre, Iain M.
Melville, Vanessa
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Melville, Vanessa
Oosthuyzen, Wilna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Oosthuyzen, Wilna
Kimmitt, Robert A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Kimmitt, Robert A.
Brown, Kayleigh E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Brown, Kayleigh E.
Kennedy, Ewan D.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Kennedy, Ewan D.
Goddard, Jane
论文数: 0引用数: 0
h-index: 0
机构:
Royal Infirm Edinburgh NHS Trust, Dept Renal Med, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Goddard, Jane
Webb, David J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
机构:
Royal Infirm Edinburgh NHS Trust, Dept Renal Med, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Dhaun, Neeraj
Moorhouse, Rebecca
论文数: 0引用数: 0
h-index: 0
机构:Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Moorhouse, Rebecca
MacIntyre, Iain M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
MacIntyre, Iain M.
Melville, Vanessa
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Melville, Vanessa
Oosthuyzen, Wilna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Oosthuyzen, Wilna
Kimmitt, Robert A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Kimmitt, Robert A.
Brown, Kayleigh E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Brown, Kayleigh E.
Kennedy, Ewan D.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Kennedy, Ewan D.
Goddard, Jane
论文数: 0引用数: 0
h-index: 0
机构:
Royal Infirm Edinburgh NHS Trust, Dept Renal Med, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland
Goddard, Jane
Webb, David J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, ScotlandUniv Edinburgh, BHF Ctr Res Excellence, Queens Med Res Inst, British Heart Fdn,Ctr Res Excellence, Edinburgh, Midlothian, Scotland