Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine

被引：65

作者：

Han, Sufang ^{[1
]}

Xiao, Zhifeng ^{[1
]}

Li, Xing ^{[1
]}

Zhao, Huan ^{[2
]}

Wang, Bin ^{[1
]}

Qiu, Zhixue ^{[2
]}

Li, Zhi ^{[2
]}

Mei, Xin ^{[2
]}

Xu, Bai ^{[3
]}

Fan, Caixia ^{[3
]}

Chen, Bing ^{[1
]}

Han, Jin ^{[1
]}

Gu, Yanzheng ^{[2
]}

Yang, Huilin ^{[2
]}

Shi, Qin ^{[2
]}

Dai, Jianwu ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100080, Peoples R China

[2] Soochow Univ, Inst Orthopaed, Affiliated Hosp 1, Dept Orthopaed, Suzhou 215006, Peoples R China

[3] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Div Nanobiomed, Key Lab Nanobio Interface Res, Suzhou 215123, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2018年 / 61卷 / 01期

基金：

中国国家自然科学基金;

关键词：

spinal cord injury; hPMSCs; LOCS; canine; regeneration; NERVE REGENERATION; PROLIFERATION; PROMOTES; DIFFERENTIATION; STRATEGIES; SURVIVAL; REPAIR;

D O I：

10.1007/s11427-016-9002-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Traumatic spinal cord injury (SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold (LOCS) and human placenta-derived mesenchymal stem cells (hPMSCs) when transplanted into completely transected beagle dogs. After 36 weeks observation, we found that LOCS+hPMSCs implants promoted better hindlimb locomotor recovery than was observed in the non-treatment (control) group and LOCS group. Histological analysis showed that the regenerated tissue after treatment was well integrated with the host tissue, and dramatically reduced the volume of cystic and chondroitin sulfate proteoglycans (CSPGs) expression. Furthermore, the LOCS+hPMSCs group also showed more neuron-specific beta III-tubulin (Tuj-1)- and NeuN-positive neurons in the lesion area, as well as axonal regeneration, remyelination and synapse formation in the lesion site. Additionally, dogs in the LOCS+hPMSCs group experienced enhanced sprouting of both ascending (CGRP-positive) sensory fibers and descending (5-HT- and TH-positive) motor fibers at the lesion area. All these data together suggested that the combined treatment had beneficial effects on neuronal regeneration and functional improvement in a canine complete transection model. Therefore, LOCS+hPMSCs implantation holds a great promise for bridging the nerve defect and may be clinically useful in the near future.

引用

页码：2 / 13

页数：12

共 46 条

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