Oct-2-en-4-ynoyl-CoA as a specific inhibitor of acyl-CoA oxidase

被引：10

作者：

Zeng, Jia ^{[1
,2
]}

Wu, Long ^{[1
]}

Zhang, Xiaojian ^{[2
]}

Liu, Yuandong ^{[2
]}

Deng, Guisheng ^{[1
]}

Li, Ding ^{[1
]}

机构：

[1] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China

[2] Cent S Univ, Dept Bioengn, Changsha 410083, Hunan, Peoples R China

来源：

ORGANIC LETTERS | 2008年 / 10卷 / 19期

关键词：

D O I：

10.1021/ol801571n

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Oct-2-en-4-ynoyl-CoA was found to be a specific inhibitor of acyl-CoA oxidase in fatty acid oxidation in peroxisomes that has no inhibitory effect on acyl-CoA dehydrogenase in mitochondria. The inhibition reaction involves a nucleophilic attack of Glu421 to the delta carbon of the inhibitor. The result indicates that acyl-CoA oxidase and acyl-CoA dehydrogenase have certain differences in active-site structure, which makes it possible to control fatty acid oxidation selectively in either mitochondria or peroxisomes with different enzyme inhibitors.

引用

页码：4287 / 4290

页数：4

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