Antioxidants increase number of progenitor endothelial cells through multiple gene expression pathways

被引：34

作者：

Fiorito, Carmela ^{[2
,3
,4
]}

Rienzo, Monica ^{[2
,3
]}

Crimi, Ettore ^{[5
,6
]}

Rossiello, Raffaele ^{[1
,7
]}

Balestrieri, Maria Luisa ^{[1
,7
]}

Casamassimi, Amelia ^{[2
,3
]}

Muto, Francesco ^{[2
,3
]}

Grimaldi, Vincenzo ^{[2
,3
]}

Giovane, Alfonso ^{[1
,7
]}

Farzati, Bartolomeo ^{[2
,3
]}

Mancini, Francesco P. ^{[8
]}

Napoli, Claudio ^{[2
,3
]}

机构：

[1] Univ Naples 2, Dept Biochem & Biophys, Sch Med 1, I-80138 Naples, Italy

[2] Univ Naples 2, Dept Gen Pathol, I-80138 Naples, Italy

[3] Univ Naples 2, Excellence Res Ctr Cardiovasc Dis, Div Clin Pathol, I-80138 Naples, Italy

[4] IRCCS Multimedia, Milan, Italy

[5] Univ Novara, Dept Anesthesiol, Novara, Italy

[6] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Anesthesia & Crit Care, Boston, MA 02115 USA

[7] Univ Naples 2, Sch Med 1, Dept Biochem & Biophys, Naples, Italy

[8] Univ Sannio, Dept Biol & Environm Sci, Benevento, Italy

来源：

FREE RADICAL RESEARCH | 2008年 / 42卷 / 08期

关键词：

antioxidants; vitamins; endothelial progenitor cells; VEGF; microarray;

D O I：

10.1080/10715760802357057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To date, there is no report on the effect of antioxidants on endothelial progenitor cells (EPCs). This study shows that in vitro incubation of EPCs with vitamin C and E reverted the already well documented lowering effect of TNF-alpha on EPC number and increased p-p38 expression levels. In order to document major changes of gene expression levels and gain insight into signalling pathways, microarray analysis was performed and a significant variation of the expression of 5389 genes in EPCs following antioxidant treatment was detected. Also in vivo evidence is provided about the positive effect of antioxidant vitamins on EPCs, since vitamin C and E supplementation potentiated the physical training-induced increase of EPC number and VEGF levels. Together, these data indicate that antioxidant treatment ameliorates EPC number and causes major changes of gene expression within these cells in vitro. Furthermore, concomitant antioxidant supplementation and physical training in vivo raised the levels of circulating EPCs and serum VEGF more than physical training alone.

引用

页码：754 / 762

页数：9

共 46 条

[1] Isolation of putative progenitor endothelial cells for angiogenesis [J].