Diversity of glutamate dehydrogenase in human brain

被引：17

作者：

Burbaeva, GS ^{[1
]}

Turishcheva, MS ^{[1
]}

Vorobyeva, EA ^{[1
]}

Savushkina, OK ^{[1
]}

Tereshkina, EB ^{[1
]}

Boksha, IS ^{[1
]}

机构：

[1] RAMS, Mental Hlth Res Ctr, Neurochem Lab, Moscow 113152, Russia

来源：

PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY | 2002年 / 26卷 / 03期

关键词：

antiglutamate dehydrogenase polyclonal antibodies; glutamate dehydrogenase isoenzymes; human brain; western immunoblotting;

D O I：

10.1016/S0278-5846(01)00273-1

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Three forms of glutamate dehydrogenase (GDH, EC 1.4.1.3) are purified from human brain tissue. Two of them, named GDH I (consisting of 58 +/-1-kDa subunit) and GDH II (consisting of 56 +/- 1-kDa subunit), are readily solubilized and the third one, GDH III (consisting of 56 +/- 1-kDa subunit), is a membrane-associated (particulate bound) isoform. Kinetic constants were determined for GDH III. These GDH forms were found to differ in hydrophobicity as indicated by different affinity to Phenyl-Sepharose. All three GDH forms showed microheterogeneity on two-dimensional (2-D) gel electrophoresis. Specific polyclonal antibodies, which enable to determine the levels of immunoreactivities of all the GDH forms in human brain extracts by enzyme-chemiluminescent amplified (ECL)-Western immunoblotting, were obtained. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：427 / 435

页数：9

共 31 条

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