Production of an agonist-like monoclonal antibody to the human A2A receptor of adenosine for clinical use

被引：32

作者：

By, Youlet ^{[1
]}

Durand-Gorde, Josee-Martine ^{[1
]}

Condo, Jocelyne ^{[1
]}

Lejeune, Pierre-Jean ^{[1
]}

Mallet, Bernard ^{[1
]}

Carayon, Pierre ^{[1
]}

Guieu, Regis ^{[1
]}

Ruf, Jean ^{[1
]}

机构：

[1] Univ Mediterranee, Fac Med Timone, Lab Biochim Endocrinienne & Metab, EA3288, F-13385 Marseille 5, France

来源：

MOLECULAR IMMUNOLOGY | 2009年 / 46卷 / 03期

关键词：

Adenosine receptor; Monoclonal antibody; Peptide; Linear epitope; Agonist; BETA(2)-ADRENERGIC RECEPTOR; CELLS; EXPRESSION; FAILURE; STATE;

D O I：

10.1016/j.molimm.2008.10.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The second extracellular loop of the A(2A) receptor (A(2A)R) of adenosine Was Used to immunize mice for production of Adonis. an IgM monoclonal antibody. Adonis bound to the immunogen peptide and the native receptor in ELISA with K-D values in 6.51-12.35 nM range. It recognized a linear epitope of 7 amino acids (LFEDVVP) at the C-terminal part of the external loop. Adonis revealed a 45-kDa hand in lysate of human peripheral blood mononuclear cells in Western blotting in denaturing conditions. This served to monitor the up-regulation of the A(2A)R expression by caffeine. Adonis stimulated the cAMP production and inhibited the cell proliferation of an A(2A)R transfected stable cell line. These results confirm the immunogenicity and the functional relevance of the second extracellular loop of the A(2A)R. They suggest that Adonis may be of clinical use in various pathological situations to measure the regulation of the A(2A)R expression and to act as A(2A)R agonist drug. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：400 / 405

页数：6

共 27 条

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