Effect of bisphenol A with or without enzyme treatment on the proliferation and viability of MCF-7 cells

被引：39

作者：

Ricupito, A. ^{[2
]}

Del Pozzo, G. ^{[1
]}

Diano, N. ^{[2
,3
]}

Grano, V. ^{[2
,3
]}

Portaccio, M. ^{[2
,3
]}

Marino, M. ^{[2
,4
]}

Bolli, A. ^{[2
,4
]}

Galluzzo, P. ^{[2
,4
]}

Bontempo, P. ^{[5
]}

Mita, L. ^{[2
,5
]}

Altucci, L. ^{[2
,5
]}

Mita, D. G. ^{[1
,2
,3
]}

机构：

[1] Inst Genet & Biophys ABT, I-80131 Naples, Italy

[2] Natl Inst Biosyst & Biostruct INBB, I-00136 Rome, Italy

[3] Univ Naples 2, Dept Expt Med, I-80138 Naples, Italy

[4] Univ RomaTre, Dept Biol, I-00146 Rome, Italy

[5] Univ Naples 2, Dept Gen Pathol, I-80138 Naples, Italy

来源：

ENVIRONMENT INTERNATIONAL | 2009年 / 35卷 / 01期

关键词：

MCF-7; BPA; Bioremediation; Proliferation index; Viability index; NONISOTHERMAL BIOREACTORS; PERINATAL EXPOSURE; MATTER TRANSPORT; FEMALE MOUSE; WASTE-WATERS; ESTROGENICITY; LACCASE; DETOXIFICATION; MECHANISMS; ESTRADIOL;

D O I：

10.1016/j.envint.2008.05.011

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Recently, aqueous solutions polluted by BPA have been bioremediated by us using laccase immobilized on hydrophobic membranes in non-isothermal bioreactors. BPA degradation was checked using analytical methods. To assess in vitro the occurred bioremediation, the proliferation and viability indexes of MCF-7 cells incubated in the presence of aqueous solutions of BPA, or of enzyme-treated BPA solutions, have been measured as a function of the initial BPA concentration. The results demonstrated that: i) at each initial BPA concentration used, both the proliferation and viability indexes are a function of the duration of enzyme treatment: ii) proliferation and viability are uncoupled biological processes with respect to BPA enzyme treatment. Non-isothermal bioreactors are a useful tool for the bioremediation of aqueous solutions polluted by BPA, which is an example of an endocrine disruptor that belongs to the alkyl phenol family. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：21 / 26

页数：6

共 34 条

[1] Effects of bisphenol A on adult male mouse fertility [J].