Quantitative determination of non-starch polysaccharides in foods using Gas Chromatography with flame ionization detection

被引：21

作者：

Ma, Jun ^{[1
]}

Adler, Lewis ^{[2
]}

Srzednicki, George ^{[1
]}

Arcot, Jayashree ^{[1
,3
]}

机构：

[1] UNSW Australia, Sch Chem Engn, Food Sci & Technol Grp, Sydney, NSW 2052, Australia

[2] UNSW Australia, Mark Wainwright Analyt Ctr, Bioanalyt Mass Spectrometry Facil, Sydney, NSW 2052, Australia

[3] UNSW Australia, Sch Chem Engn, ARC Training Ctr Adv Technol Food Mfg, Sydney, NSW 2052, Australia

来源：

FOOD CHEMISTRY | 2017年 / 220卷

关键词：

Gas Chromatography; Non-starch polysaccharides; Banana pseudostem; DIETARY FIBER; BETA-GLUCAN; PSEUDO-STEM; BANANA; SUGARS;

D O I：

10.1016/j.foodchem.2016.09.206

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A gas chromatographic method was developed for the quantitative determination of the monosaccharides of the soluble, insoluble fractions and the total non-starch polysaccharides (NSPs) content in foods with fat contents of less than 5%. Sample preparation involved enzymatic removal of starch and acid hydrolysis of the NSP to their constituent sugars. The alditol acetate derivatives were analysed on a wide bore capillary column with detection by flame ionization. The method was accurate, with recovery of spiked samples between 93.6% and 102.7% for intra-day analysis and between 93.2% and 104.7% for inter-day analysis. Repeatability was excellent; RSD values from 0.1 to 4.4% and 0.2 to 5.7% were observed from intra-day analysis and inter-day analysis, respectively. The peaks for all neutral sugars were sharp and separation was at baseline resolution with no interfering or co-eluting peaks. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：100 / 107

页数：8

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