A beginner's guide to atomic force microscopy probing for cell mechanics

被引：137

作者：

Gavara, Nuria ^{[1
]}

机构：

[1] Queen Mary Univ London, Sch Engn & Mat Sci, Mile End Rd, London E1 3NS, England

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2017年 / 80卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

atomic force microscopy; cell mechanics; mechanobiology; VISCOELASTIC PROPERTIES; ELASTIC-MODULI; ADHESION; CONTACT; DEFORMATION; STIFFNESS; SAMPLES; SHAPE;

D O I：

10.1002/jemt.22776

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Atomic Force microscopy (AFM) is becoming a prevalent tool in cell biology and biomedical studies, especially those focusing on the mechanical properties of cells and tissues. The newest generation of bio-AFMs combine ease of use and seamless integration with live-cell epifluorescence or more advanced optical microscopies. As a unique feature with respect to other bionanotools, AFM provides nanometer-resolution maps for cell topography, stiffness, viscoelasticity, and adhesion, often overlaid with matching optical images of the probed cells. This review is intended for those about to embark in the use of bio-AFMs, and aims to assist them in designing an experiment to measure the mechanical properties of adherent cells. In addition to describing the main steps in a typical cell mechanics protocol and explaining how data is analysed, this review will also discuss some of the relevant contact mechanics models available and how they have been used to characterize specific features of cellular and biological samples.

引用

页码：75 / 84

页数：10

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