Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type

被引：174

作者：

Boldog, Eszter ^{[1
]}

Bakken, Trygve E. ^{[2
]}

Hodge, Rebecca D. ^{[2
]}

Novotny, Mark ^{[3
]}

Aevermann, Brian D. ^{[3
]}

Baka, Judith ^{[1
]}

Borde, Sandor ^{[1
]}

Close, Jennie L. ^{[2
]}

Diez-Fuertes, Francisco ^{[3
]}

Ding, Song-Lin ^{[2
]}

Farago, Nora ^{[1
]}

Kocsis, Agnes K. ^{[1
]}

Kovacs, Balazs ^{[1
]}

Maltzer, Zoe ^{[2
]}

McCorrison, Jamison M. ^{[3
]}

Miller, Jeremy A. ^{[2
]}

Molnar, Gabor ^{[1
]}

Olah, Gaspar ^{[1
]}

Ozsvar, Attila ^{[1
]}

Rozsa, Marton ^{[1
]}

Shehata, Soraya I. ^{[2
]}

Smith, Kimberly A. ^{[2
]}

Sunkin, Susan M. ^{[2
]}

Tran, Danny N. ^{[3
]}

Venepally, Pratap ^{[3
]}

Wall, Abby ^{[2
]}

Puskas, Laszlo G. ^{[4
]}

Barzo, Pal ^{[5
]}

Steemers, Frank J. ^{[6
]}

Schork, Nicholas J. ^{[3
]}

Scheuermann, Richard H. ^{[3
,7
]}

Lasken, Roger S. ^{[3
]}

Lein, Ed S. ^{[2
]}

Tamas, Gabor ^{[1
]}

机构：

[1] Univ Szeged, Dept Anat Physiol & Neurosci, MTA SZTE Res Grp Cort Microcircuits, Szeged, Hungary

[2] Allen Inst Brain Sci, Seattle, WA 98109 USA

[3] J Craig Venter Inst, La Jolla, CA USA

[4] Hungarian Acad Sci, Dept Genet, Biol Res Ctr, Lab Funct Genom, Szeged, Hungary

[5] Univ Szeged, Dept Neurosurg, Szeged, Hungary

[6] Illumina Inc, San Diego, CA USA

[7] Univ Calif San Diego, Dept Pathol, La Jolla, CA 92093 USA

来源：

NATURE NEUROSCIENCE | 2018年 / 21卷 / 09期

关键词：

RNA-SEQ; SYNAPTIC CONNECTIONS; QUALITY-CONTROL; EXPRESSION ANALYSIS; VISUAL-CORTEX; ANIMAL-MODELS; SINGLE NUCLEI; NEURONS; INTERNEURONS; DIVERSITY;

D O I：

10.1038/s41593-018-0205-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We describe convergent evidence from transcriptomics, morphology, and physiology for a specialized GABAergic neuron subtype in human cortex. Using unbiased single-nucleus RNA sequencing, we identify ten GABAergic interneuron subtypes with combinatorial gene signatures in human cortical layer 1 and characterize a group of human interneurons with anatomical features never described in rodents, having large 'rosehip'-like axonal boutons and compact arborization. These rosehip cells show an immunohistochemical profile (GAD1(+)CCK(+), CNR1(-)SST(-)CALB2(-)PVALB(-)) matching a single transcriptomically defined cell type whose specific molecular marker signature is not seen in mouse cortex. Rosehip cells in layer 1 make homotypic gap junctions, predominantly target apical dendritic shafts of layer 3 pyramidal neurons, and inhibit backpropagating pyramidal action potentials in microdomains of the dendritic tuft. These cells are therefore positioned for potent local control of distal dendritic computation in cortical pyramidal neurons.

引用

页码：1185 / +

页数：15

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