Generation of Aptamers with an Expanded Chemical Repertoire

被引：85
作者：
Diafa, Stella [1 ]
Hollenstein, Marcel [1 ]
机构：
[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland
来源：
MOLECULES | 2015年 / 20卷 / 09期
基金：
瑞士国家科学基金会;
关键词：
aptamers; modified nucleoside triphosphates; polymerases; SELEX; therapeutic oligonucleotides; chemically modified nucleic acids; synthetic genetic polymers; IN-VITRO SELECTION; IMMUNODEFICIENCY-VIRUS TYPE-1; MODIFIED DNA APTAMERS; HIGH-AFFINITY; RNA APTAMERS; DEOXYNUCLEOSIDE TRIPHOSPHATES; COMBINATORIAL SELECTION; DIRECTED EVOLUTION; NUCLEIC-ACIDS; POLYMERASE INCORPORATION;
D O I：
10.3390/molecules200916643
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The enzymatic co-polymerization of modified nucleoside triphosphates (dN*TPs and N*TPs) is a versatile method for the expansion and exploration of expanded chemical space in SELEX and related combinatorial methods of in vitro selection. This strategy can be exploited to generate aptamers with improved or hitherto unknown properties. In this review, we discuss the nature of the functionalities appended to nucleoside triphosphates and their impact on selection experiments. The properties of the resulting modified aptamers will be described, particularly those integrated in the fields of biomolecular diagnostics, therapeutics, and in the expansion of genetic systems (XNAs).
引用
收藏
页码：16643 / 16671
页数：29
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