Isolation and characterization of Xanthobacter polyaromaticivorans sp nov 127W that degrades polycyclic and heterocyclic aromatic compounds under extremely low oxygen conditions

被引:28
作者
Hirano, SI
Kitauchi, F
Haruki, M
Imanaka, T
Morikawa, M [1 ]
Kanaya, S
机构
[1] Osaka Univ, Grad Sch Engn, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan
[2] Kyoto Univ, Grad Sch Engn, Dept Synth Chem & Biol Chem, Kyoto 6158510, Japan
关键词
Xanthobacter polyaromaticivornas; polycyclic aromatic hydrocarbons; heterocyclic aromatic compounds; extremely low oxygen; degradation;
D O I
10.1271/bbb.68.557
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two bacterial strains, 127W and T102, were isolated from anoxic crude oil tank sludge as effective degraders of dibenzothiophene (DBT), a model sulfur containing heterocyclic aromatic compound in crude oil. Strain 127W was more tolerant to oxygen limitation than T102 and was capable of degrading two- and three-ring polycyclic and heterocyclic aromatic compounds under both aerobic and low oxygen conditions. Strain 127W degraded 0.082, 0.055, and 0.064 mm of DBT, naphthalene, and anthracene, respectively, in one week with dissolved oxygen </=0.2ppm (0.006mm). Degradation by 127W cell-free extracts for DBT was increased by addition of sodium hydrogencarbonate under this oxygen concentration. Phylogenetic analysis of the 16S rRNA gene sequence and physiological characteristics indicate that the strains 127W and T102 belong to new species of the genus Xanthobacter and Pseudomonas stutzeri, respectively. We propose X. polyaromaticivorans sp. nov. 127W.
引用
收藏
页码:557 / 564
页数:8
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