Sphingosylphosphorylcholine induces mitochondria-mediated apoptosis in neuro 2a cells: Involvement of protein kinase C

被引:0
|
作者
Miguel, Begona G. [1 ]
Fernandez, Inmaculada [1 ]
Toboso, Inmaculada [1 ]
Agudo-Lopez, Alba
Catalan, Edgardo [2 ]
Martinez, Ana M. [1 ]
机构
[1] Univ Complutense Madrid, Fac Quim, Dept Bioquim & Biol Mol 1, E-28040 Madrid, Spain
[2] Univ Complutense Madrid, Dept Biol Mol, CBMSO, E-28040 Madrid, Spain
关键词
Sphingosylphosphorylcholine; apoptosis; protein kinase C; neuroblastoma neuro 2a cells;
D O I
暂无
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We demonstrate that sphingosylphosphorylcholine-mediated cell death involves the activation of different protein kinase C isozymes in different manners. Treating cells with sphingosylphosphorylcholine resulted in activation of protein kinase Ca2-, which is necessary, together with elevation of Ca2-, for sphingosylphosphorylcholine-induced apoptosis. A rapid translocation from cytosol to membrane, and a proteolytic protein kinase C delta cleavage was found, probably due to activation of caspase-3, to give a catalytically active fragment involvecl in cellular apoptosis. Moreover, sphingosyl-phosphorylcholine also induced translocation of protein kinase C zeta, resulting in an anti-apoptotic effect. To explore whether a mitochondrial pathway is involved in sphingosylphosphorylcholine-induced apoptosis, we analyzed the effect of sphingosylphosphorylcholine on cytochrome c release and caspase-3 activity. We must point out that the sphingolipid caused an increase of cytochrome c release front mitochondria to cytosol concomitantly with an increase of caspase-3 activity. Furthermore. a translocation of Bax was found, after sphingosylphosphorylcholine treatment.
引用
收藏
页码:443 / 452
页数:10
相关论文
共 50 条
  • [21] Involvement of mitochondria-mediated apoptosis in α and β-Zearalenol cytotoxicity, prevention by Crocin
    Salem, I.
    Boussabbeh, M.
    Prola, A.
    Lemaire, C.
    Bacha, H.
    Abid-Essefi, S.
    FEBS JOURNAL, 2014, 281 : 354 - 354
  • [22] Severe hyperthyroidism induces mitochondria-mediated apoptosis in rat liver
    Upadhyay, G
    Singh, R
    Kumar, A
    Kumar, S
    Kapoor, A
    Godbole, MM
    HEPATOLOGY, 2004, 39 (04) : 1120 - 1130
  • [23] Deoxynivalenol induces apoptosis in mouse thymic epithelial cells through mitochondria-mediated pathway
    Li, Daotong
    Ma, Haoran
    Ye, Yaqiong
    Ji, Changyun
    Tang, Xiaohong
    Ouyang, Dan
    Chen, Jian
    Li, Yugu
    Ma, Yongjiang
    ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2014, 38 (01) : 163 - 171
  • [24] Noscapine induces mitochondria-mediated apoptosis in human colon cancer cells in vivo and in vitro
    Yang, Zi-Rong
    Liu, Meng
    Peng, Xiu-Lan
    Lei, Xiao-Fei
    Zhang, Ji-Xiang
    Dong, Wei-Guo
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 421 (03) : 627 - 633
  • [25] Hydramethylnon induces mitochondria-mediated apoptosis in BEAS-2B human bronchial epithelial cells
    Kang, Yeon-Ho
    Jeong, Hye-Jin
    Park, Yong Joo
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2024, 492
  • [26] Microcystin-LR induces mitochondria-mediated apoptosis in human bronchial epithelial cells
    Li, Yang
    Li, Jinhui
    Huang, Hui
    Yang, Mingfeng
    Zhuang, Donggang
    Cheng, Xuemin
    Zhang, Huizhen
    Fu, Xiaoli
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2016, 12 (02) : 633 - 640
  • [27] Naja ashei venom induces mitochondria-mediated apoptosis in human colorectal cancer cells
    Antolikova, Natalia Rozman
    Kello, Martin
    Zigova, Martina
    Tischlerova, Viera
    Petrilla, Vladimir
    Pirnik, Zdenko
    Mojzisova, Gabriela
    Mojzis, Jan
    ACTA BIOCHIMICA POLONICA, 2019, 66 (02) : 207 - 213
  • [28] Celastrol Induces Mitochondria-Mediated Apoptosis in Hepatocellular Carcinoma Bel-7402 Cells
    Li, Pei-Pei
    He, Wei
    Yuan, Ping-Fan
    Song, Sha-Sha
    Lu, Jing-Tao
    Wei, Wei
    AMERICAN JOURNAL OF CHINESE MEDICINE, 2015, 43 (01): : 137 - 148
  • [29] Plumbagin induces the apoptosis of human tongue carcinoma cells through the mitochondria-mediated pathway
    Qiu, Jia-xuan
    He, Yuan-qiao
    Wang, Yong
    Xu, Ru-liang
    Qin, You
    Shen, Xiang
    Zhou, Shu-feng
    Mao, Zong-fu
    MEDICAL SCIENCE MONITOR BASIC RESEARCH, 2013, 19 : 228 - 236
  • [30] Carbon disulfide induces mitochondria-mediated apoptosis in Sertoli-germ cells coculture
    Wang, Wei
    Zhang, Zhen
    Guo, Yinsheng
    Dong, Yu
    Huang, Xiaoyu
    Zhou, Yijun
    Chen, Guoyuan
    MOLECULAR & CELLULAR TOXICOLOGY, 2015, 11 (02) : 175 - 185