Sphingosylphosphorylcholine induces mitochondria-mediated apoptosis in neuro 2a cells: Involvement of protein kinase C

被引:0
|
作者
Miguel, Begona G. [1 ]
Fernandez, Inmaculada [1 ]
Toboso, Inmaculada [1 ]
Agudo-Lopez, Alba
Catalan, Edgardo [2 ]
Martinez, Ana M. [1 ]
机构
[1] Univ Complutense Madrid, Fac Quim, Dept Bioquim & Biol Mol 1, E-28040 Madrid, Spain
[2] Univ Complutense Madrid, Dept Biol Mol, CBMSO, E-28040 Madrid, Spain
关键词
Sphingosylphosphorylcholine; apoptosis; protein kinase C; neuroblastoma neuro 2a cells;
D O I
暂无
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We demonstrate that sphingosylphosphorylcholine-mediated cell death involves the activation of different protein kinase C isozymes in different manners. Treating cells with sphingosylphosphorylcholine resulted in activation of protein kinase Ca2-, which is necessary, together with elevation of Ca2-, for sphingosylphosphorylcholine-induced apoptosis. A rapid translocation from cytosol to membrane, and a proteolytic protein kinase C delta cleavage was found, probably due to activation of caspase-3, to give a catalytically active fragment involvecl in cellular apoptosis. Moreover, sphingosyl-phosphorylcholine also induced translocation of protein kinase C zeta, resulting in an anti-apoptotic effect. To explore whether a mitochondrial pathway is involved in sphingosylphosphorylcholine-induced apoptosis, we analyzed the effect of sphingosylphosphorylcholine on cytochrome c release and caspase-3 activity. We must point out that the sphingolipid caused an increase of cytochrome c release front mitochondria to cytosol concomitantly with an increase of caspase-3 activity. Furthermore. a translocation of Bax was found, after sphingosylphosphorylcholine treatment.
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页码:443 / 452
页数:10
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