Pyranose 2-oxidase (P2O): Production from Trametes versicolor in Stirred Tank Reactor and its Partial Characterization

被引：1

作者：

Pazarlioglu, Nurdan Kasikara ^{[1
]}

Akkaya, Alper ^{[1
]}

Tahsinsoy, Dilek ^{[1
]}

机构：

[1] Ege Univ, Dept Biochem, Fac Sci, TR-35100 Bornova, Turkey

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2009年 / 39卷 / 01期

关键词：

Partial characterization; Production conditions; Pyranose; 2-oxidase; Stirred tank reactor; Trametes versicolor; PHANEROCHAETE-CHRYSOSPORIUM; CORIOLUS-VERSICOLOR; CRYSTAL-STRUCTURE; OXIDASE; PURIFICATION; ENZYME;

D O I：

10.1080/10826060802589569

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimization of pyranose-2-oxidase (P2O) production conditions from Trametes versicolor was carried out in shaking cultures containing glucose, malt, and yeast extracts; the optimum concentration values were found to be 1.5% glucose, 1.0% yeast extract, and 1.0% malt extract, pH 5.0, temperature, 26 degrees C, and agitation rate 150 rpm. For the first time, P2O production was also carried out in a stirred tank reactor (STR) with 2.2 L working volume in the optimized medium composition, and biomass, P2O activity, protein, nitrogen and glucose concentrations were also monitored besides pH and dissolved oxygen (DO). In the STR, P2O activity peaked on day 9. Partial enzyme characterization occurred and optimum pH and temperature were detected as 7.0 and 37 degrees C, respectively. Km value was found to be 1.009 mM.

引用

页码：32 / 45

页数：14

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