Characterization of cockroach (Periplaneta americana) fat body phospholipase A2 activity

被引：8

作者：

Sun, D ^{[1
]}

Steele, JE ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Zool, London, ON N6A 5B7, Canada

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 2002年 / 49卷 / 03期

关键词：

fat body; Periplaneta americana; phospholipase A(2); phospholipid;

D O I：

10.1002/arch.10014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A phospholipase has been identified in the fat body of the American cockroach, Periplaneta americana, which removes fatty acid from the sn-2 acyl position of on artificial substrate, The enzyme has been characterized using a crude preparation obtained by low-speed centrifugation of the homogenized tissue. With 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine as the substrate, the K-m has been estimated to be 1.17 muM and the v(max) 113.5 pmol/min/mg protein. The phospholipase has a pH optimum close to 7 and shows maximal activity at 50degreesC. Activity of the phospholipase has been determined in cytosolic and plasma membrane fractions, The specific activity of the latter fraction is approximately twice that of the cytosol. The enzyme in both fractions is Ca2+-independent. (C) 2002 Wiley-Liss, Inc.

引用

页码：149 / 157

页数：9

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