In vivo cytometry: A spectrum of possibilites

被引:33
作者
Chung, A
Karlan, S
Lindsley, E
Wachsmann-Hogiu, S
Farkas, DL
机构
[1] Cedars Sinai Med Ctr, Minimally Invas Sug Technol Inst, Los Angeles, CA 90048 USA
[2] Cedars Sinai Med Ctr, Dept Surg, Los Angeles, CA 90048 USA
关键词
optical diagnostics; unstained specimens; in vivo cytometry; spectral imaging; elastic scattering imaging endoscopy;
D O I
10.1002/cyto.a.20220
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: We investigate whether optical imaging can reliably detect abnormalities in tissue, in a range of specimens (live cells in vitro; fixed, fresh ex-vivo and in Vivo tissue), without the use of added contrast agents, and review our promising spectral methods for achieving quantitative, real-time, high resolution intrasurgical optical diagnostics. Methods: We use reflectance, fluorescence, two-photon, and Mic scattering imaging, performed with instrumentation we developed or modified, to detect intrinsic tissue signatures. Emphasis is on spectral/hyperspectral imaging approaches allowing the equivalent of in vivo pathology. Results: With experimental focus on unstained specimens, we demonstrate the ability to segment tissue images for cancer detection. Spectral reflectance imaging, Coupled with advanced analysis, typically yields 90% specificity and sensitivity. Autofluorescence is also shown to be diagnostically useful, with lymph nodes results highlighted here. Elastic scattering hyperspectral imaging endoscopy, using a new instrument we designed and built, shows promise in bronchoscopic detection of dysplasia and early cancer in patients. Conclusions: The results demonstrate that advanced optical imaging can detect and localize cellular signatures of cancer in real-time, in vivo, without the use of contrast agents, in animals and humans. This is an important step towards tight spatio-temporal coupling between such detection and clinical intervention. (c) 2006 international Society for Analytical Cytology.
引用
收藏
页码:142 / 146
页数:5
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