Covalent Immobilization of Cellulase Using Magnetic Poly(ionic liquid) Support: Improvement of the Enzyme Activity and Stability

被引：98
|
作者：
Hosseini, Seyed Hassan [1 ]
Hosseini, Seyedeh Ameneh [1 ]
Zohreh, Nasrin [2 ]
Yaghoubi, Mahshid [3 ]
Pourjavadi, Ali [3 ]
机构：
[1] Univ Sci & Technol Mazandaran, Dept Chem Engn, Behshahr, Iran
[2] Univ Qom, Fac Sci, Dept Chem, Qom, Iran
[3] Sharif Univ Technol, Dept Chem, Polymer Res Lab, Tehran, Iran
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 04期
关键词：
cellulase; covalent immobilization; magnetic support; ionic liquid; MESOPOROUS SILICA; SUPERPARAMAGNETIC NANOPARTICLES; BIOETHANOL PRODUCTION; PHYSICAL ADSORPTION; BACILLUS-CIRCULANS; BETA-GALACTOSIDASE; EPOXY SUPPORTS; HYDROLYSIS; CHITOSAN; LIPASE;
D O I：
10.1021/acs.jafc.7b03922
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A magnetic nanocomposite was prepared by entrapment of Fe3O4 nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization of cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme, and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structure of support was characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM, and DLS. The activity and stability of immobilized cellulase were investigated in the prepared support. The results showed that the ionic surface and covalent binding of enzyme onto the support improved the activity, thermal stability, and reusability of cellulase compared to free cellulase.
引用
收藏
页码：789 / 798
页数：10
相关论文
共 38 条
  • [21] Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability
    Yabuki, Soichi
    Hirata, Yoshiki
    Sato, Yuko
    Iijima, Seiichiro
    ANALYTICAL SCIENCES, 2012, 28 (04) : 373 - 377
  • [22] Immobilization of enzymes using non-ionic colloidal liquid aphrons (CLAs): Surface and enzyme effects
    Ward, Keeran
    Xi, Jingshu
    Stuckey, David C.
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2015, 136 : 424 - 430
  • [23] Improving the performance of a glucose biosensor using an ionic liquid for enzyme immobilization. On the chemical interaction between the biomolecule, the ionic liquid and the cross-linking agent
    Galhardo, Kelly S.
    Torresi, Roberto M.
    Cordoba de Torresi, Susana I.
    ELECTROCHIMICA ACTA, 2012, 73 : 123 - 128
  • [24] Cooperativity of covalent attachment and ion exchange on alcalase immobilization using glutaraldehyde chemistry: Enzyme stabilization and improved proteolytic activity
    Ait Braham, Sabrina
    Hussain, Fouzia
    Morellon-Sterling, Roberto
    Kamal, Shagufta
    Kornecki, Jakub F.
    Barbosa, Oveimar
    Kati, Djamel Edine
    Fernandez-Lafuente, Roberto
    BIOTECHNOLOGY PROGRESS, 2019, 35 (02)
  • [25] Influence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme
    Rasouli, N.
    Sohrabi, N.
    Zamani, M.
    PHYSICAL CHEMISTRY RESEARCH, 2016, 4 (02): : 271 - 283
  • [26] Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability
    Liu, Dong-Mei
    Chen, Juan
    Shi, Yan-Ping
    ANALYTICA CHIMICA ACTA, 2018, 1006 : 90 - 98
  • [27] Branched poly(ionic liquid)-modified magnetic nanoparticles as a nitrogen-rich and hydrophilic support for stabilization of Pd nanoparticles and exploring its catalytic activity
    Gholinejad, Mohammad
    Zareh, Fatemeh
    Bagheri, Hossein
    Nikfarjam, Nasser
    Sansano, Jose M.
    RESEARCH ON CHEMICAL INTERMEDIATES, 2024, 50 (06) : 2721 - 2741
  • [28] Promoting laccase sensing activity for catechol detection using LBL assemblies of chitosan/ionic liquid/phthalocyanine as immobilization surfaces
    Salvo-Comino, C.
    Garcia-Hernandez, C.
    Garcia-Cabezon, C.
    Rodriguez-Mendez, M. L.
    BIOELECTROCHEMISTRY, 2020, 132
  • [29] Microwave-Assisted Lipase Covalent Immobilization and Improved Its Thermal Stability Using Alcohols to Quench Excessive Activated Groups on Support Surface
    Du, Zhiqiang
    Wang, Anming
    Zhou, Cheng
    Zhu, Shemin
    Shen, Shubao
    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 2009, 42 (06) : 441 - 446
  • [30] Recovery of chymotrypsin using magnetic particles and aqueous micellar two-phase systems: Influence of non-ionic surfactants on enzyme activity
    Paulus, Anja
    Morhardt, Christian
    Lehle, Nicolas
    Franzreb, Matthias
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2014, 110 : 165 - 170
1234