Covalent Immobilization of Cellulase Using Magnetic Poly(ionic liquid) Support: Improvement of the Enzyme Activity and Stability

被引：98
|
作者：
Hosseini, Seyed Hassan [1 ]
Hosseini, Seyedeh Ameneh [1 ]
Zohreh, Nasrin [2 ]
Yaghoubi, Mahshid [3 ]
Pourjavadi, Ali [3 ]
机构：
[1] Univ Sci & Technol Mazandaran, Dept Chem Engn, Behshahr, Iran
[2] Univ Qom, Fac Sci, Dept Chem, Qom, Iran
[3] Sharif Univ Technol, Dept Chem, Polymer Res Lab, Tehran, Iran
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 04期
关键词：
cellulase; covalent immobilization; magnetic support; ionic liquid; MESOPOROUS SILICA; SUPERPARAMAGNETIC NANOPARTICLES; BIOETHANOL PRODUCTION; PHYSICAL ADSORPTION; BACILLUS-CIRCULANS; BETA-GALACTOSIDASE; EPOXY SUPPORTS; HYDROLYSIS; CHITOSAN; LIPASE;
D O I：
10.1021/acs.jafc.7b03922
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A magnetic nanocomposite was prepared by entrapment of Fe3O4 nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization of cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme, and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structure of support was characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM, and DLS. The activity and stability of immobilized cellulase were investigated in the prepared support. The results showed that the ionic surface and covalent binding of enzyme onto the support improved the activity, thermal stability, and reusability of cellulase compared to free cellulase.
引用
收藏
页码：789 / 798
页数：10
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