A differentially methylated region of the DAZ1 gene in spermatic and somatic cells

被引：8

作者：

Li, ZX

Ma, X ^{[1
]}

Wang, ZH

机构：

[1] Peking Union Med Coll, Dept Genet, Beijing 100730, Peoples R China

[2] Natl Res Inst Family Planning, Dept Genet, Beijing 100081, Peoples R China

来源：

ASIAN JOURNAL OF ANDROLOGY | 2006年 / 8卷 / 01期

关键词：

DAZ1; gene; DNA methylation; CpG island; methylated region; spermatogenesis;

D O I：

10.1111/j.1745-7262.2006.00069.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Aim: To investigate the methylation status of the deleted in azoospermia 1 (DAZ1) gene promoter region in different cell types. Methods: Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing. Results: The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm: it was methylated in leukocytes, but unmethylated in sperm. Conclusion: A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues.

引用

页码：61 / 67

页数：7