A differentially methylated region of the DAZ1 gene in spermatic and somatic cells

被引：8
|
作者：
Li, ZX
Ma, X [1 ]
Wang, ZH
机构：
[1] Peking Union Med Coll, Dept Genet, Beijing 100730, Peoples R China
[2] Natl Res Inst Family Planning, Dept Genet, Beijing 100081, Peoples R China
来源：
ASIAN JOURNAL OF ANDROLOGY | 2006年 / 8卷 / 01期
关键词：
DAZ1; gene; DNA methylation; CpG island; methylated region; spermatogenesis;
D O I：
10.1111/j.1745-7262.2006.00069.x
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
Aim: To investigate the methylation status of the deleted in azoospermia 1 (DAZ1) gene promoter region in different cell types. Methods: Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing. Results: The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm: it was methylated in leukocytes, but unmethylated in sperm. Conclusion: A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues.
引用
收藏
页码：61 / 67
页数：7
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