A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

被引：193
作者：
Attard, Everaldo [1 ]
机构：
[1] Univ Malta, Inst Earth Syst, Div Rural Sci & Food Syst, Msida 2080, Malta
来源：
CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2013年 / 8卷 / 01期
关键词：
UV-VIS spectrophotometry; Microplate reader; Methanolic extracts; Tannic acid; OLEA-EUROPAEA L; ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; VITAMIN-C; FLAVONOIDS; ACIDS;
D O I：
10.2478/s11535-012-0107-3
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Several methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228 +/- 0.187x10(3) M-1 cm(-1). The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.
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页码：48 / 53
页数：6
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