STARCH SYNTHASE5, a Noncanonical Starch Synthase-Like Protein, Promotes Starch Granule Initiation in Arabidopsis

被引：59

作者：

Abt, Melanie R. ^{[1
]}

Pfister, Barbara ^{[1
]}

Sharma, Mayank ^{[1
]}

Eicke, Simona ^{[1
]}

Burgy, Leo ^{[1
]}

Neale, Isabel ^{[1
,2
]}

Seung, David ^{[1
,3
]}

Zeeman, Samuel C. ^{[1
]}

机构：

[1] Swiss Fed Inst Technol Zurich, Inst Plant Mol Biol, CH-8092 Zurich, Switzerland

[2] Univ Cambridge, St Johns Coll, Cambridge CB2 1TP, England

[3] John Innes Ctr, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

来源：

PLANT CELL | 2020年 / 32卷 / 08期

基金：

瑞士国家科学基金会;

关键词：

ESCHERICHIA-COLI; CRYSTAL-STRUCTURES; DEGRADATION; MORPHOLOGY; SEQUENCE; MUTANTS; DOMAIN; IV; LOCALIZATION; METABOLISM;

D O I：

10.1105/tpc.19.00946

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A widely conserved but noncatalytic starch synthase-like protein interacts with the known granule-initiating factor MRC and regulates the number of starch granules formed in chloroplasts. What determines the number of starch granules in plastids is an enigmatic aspect of starch metabolism. Several structurally and functionally diverse proteins have been implicated in the granule initiation process in Arabidopsis (Arabidopsis thaliana), with each protein exerting a varying degree of influence. Here, we show that a conserved starch synthase-like protein, STARCH SYNTHASE5 (SS5), regulates the number of starch granules that form in Arabidopsis chloroplasts. Among the starch synthases, SS5 is most closely related to SS4, a major determinant of granule initiation and morphology. However, unlike SS4 and the other starch synthases, SS5 is a noncanonical isoform that lacks catalytic glycosyltransferase activity. Nevertheless, loss of SS5 reduces starch granule numbers that form per chloroplast in Arabidopsis, and ss5 mutant starch granules are larger than wild-type granules. Like SS4, SS5 has a conserved putative surface binding site for glucans and also interacts with MYOSIN-RESEMBLING CHLOROPLAST PROTEIN, a proposed structural protein influential in starch granule initiation. Phenotypic analysis of a suite of double mutants lacking both SS5 and other proteins implicated in starch granule initiation allows us to propose how SS5 may act in this process.

引用

页码：2543 / 2565

页数：23

共 71 条

[11] Predicting subcellular localization of proteins based on their N-terminal amino acid sequence [J].

Emanuelsson, O ;

Nielsen, H ;

Brunak, S ;

von Heijne, G .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 300 (04) :1005-1016

[12] ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites [J].

Emanuelsson, O ;

Nielsen, H ;

Von Heijne, G .

PROTEIN SCIENCE, 1999, 8 (05) :978-984

[13]

FURUKAWA K, 1993, J BIOL CHEM, V268, P23837

[14] Involvement of lysine-193 of the conserved "K-T-G-G" motif in the catalysis of maize starch synthase IIa [J].

Gao, Z ;

Keeling, P ;

Shibles, R ;

Guan, HP .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2004, 427 (01) :1-7

[15] USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products [J].

Geu-Flores, Fernando ;

Nour-Eldin, Hussam H. ;

Nielsen, Morten T. ;

Halkier, Barbara A. .

NUCLEIC ACIDS RESEARCH, 2007, 35 (07)

[16] Disruptions in valine degradation affect seed development and germination in Arabidopsis [J].

Gipson, Andrew B. ;

Morton, Kyla J. ;

Rhee, Rachel J. ;

Simo, Szabolcs ;

Clayton, Jack A. ;

Perrett, Morgan E. ;

Binkley, Christiana G. ;

Jensen, Erika L. ;

Oakes, Dana L. ;

Rouhier, Matthew F. ;

Rouhier, Kerry A. .

PLANT JOURNAL, 2017, 90 (06) :1029-1039

[17] A ubiquitin-10 promoter-based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression studies [J].

Grefen, Christopher ;

Donald, Naomi ;

Hashimoto, Kenji ;

Kudla, Joerg ;

Schumacher, Karin ;

Blatt, Michael R. .

PLANT JOURNAL, 2010, 64 (02) :355-365

[18] Novel mutant alleles of the starch synthesis gene TaSSIVb-D result in the reduction of starch granule number per chloroplast in wheat [J].

Guo, Huijun ;

Liu, Yunchuan ;

Li, Xiao ;

Yan, Zhihui ;

Xie, Yongdun ;

Xiong, Hongchun ;

Zhao, Linshu ;

Gu, Jiayu ;

Zhao, Shirong ;

Liu, Luxiang .

BMC GENOMICS, 2017, 18

[19] Proteome Analysis of Potato Starch Reveals the Presence of New Starch Metabolic Proteins as Well as Multiple Protease Inhibitors [J].

Helle, Stanislas ;

Bray, Fabrice ;

Verbeke, Jeremy ;

Devassine, Stephanie ;

Courseaux, Adeline ;

Facon, Maud ;

Tokarski, Caroline ;

Rolando, Christian ;

Szydlowski, Nicolas .

FRONTIERS IN PLANT SCIENCE, 2018, 9

[20]

Hostettler C, 2011, METHODS MOL BIOL, V775, P387, DOI 10.1007/978-1-61779-237-3_21

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