Effect of astragali radix extract on lipopolysaccharide-induced inflammation in human amnion

The effects of Astragali radix extract on interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha productions, prostaglandin E-2 (PGE(2)) biosynthesis, and leuikotriene C-4 (LTC4) production from lipopolysaccharide (LPS)stimulated human amnion cells were investigated. Amnion cells produced detectable amounts of both IL-6 and TNF-a under LPS-stimulated conditions. Astragalus extract inhibited the production of 11-6. However, TNF-a production was not inhibited by the extract on L929 cytotoxicity assay. Treatment of amnion cells with LPS for up to 24h resulted in an increase in PGE2 release in a concentration- and time-dependent manner. The extract (150 mg/ml) significantly inhibited the output of PGE, by amnion cells (p <0.01). The arachidonate lipoxygenase metabolite (LTC4) was increased by LPS treatment of amnion cells. Astragalus extract (30 mg/ml) inhibited LTC4 production by approximately 65% throughout the culture period. These results suggest that Astragali radix extract may have a role in inhibiting bacterial infection-associated preterm labor by suppressing the productions of IL-6, PGE2, and LTC, by human amnion cells.