Characterization of Galectin-2 from Nile tilapia (Oreochromis niloticus) involved in the immune response to bacterial infection

被引：21

作者：

Niu, Jinzhong ^{[1
,2
,3
]}

Huang, Yu ^{[1
,2
,3
,4
]}

Niu, Jimin ^{[1
,2
,3
]}

Wang, Zhiwen ^{[1
,2
,3
]}

Tang, Jufen ^{[1
,2
,3
,4
]}

Wang, Bei ^{[1
,2
,3
,4
]}

Lu, Yishan ^{[1
,2
,3
,4
]}

Cai, Jia ^{[1
,2
,3
,4
]}

Jian, Jichang ^{[1
,2
,3
,4
]}

机构：

[1] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China

[2] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China

[3] Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China

[4] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2019年 / 87卷

基金：

中国国家自然科学基金;

关键词：

Galectin-2; Nile tilapia; Streptococcus agalactiae; Agglutination; Phagocytosis; Immune system; FUNCTIONAL-CHARACTERIZATION; BINDING LECTIN; HEAD KIDNEY; IN-VITRO; EXPRESSION; IDENTIFICATION; FISH; RECOGNITION; CONTRIBUTES; MACROPHAGES;

D O I：

10.1016/j.fsi.2019.02.026

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

galectin-2 plays important roles in innate and adaptive immunity. In this study, galectin-2 (OnGal 2) was identified from Nile tilapia (Oreochromis niloticus). Its tissue distribution and expression patterns following bacterial infection were also investigated. OnGal-2 is widely distributed in various tissues of healthy tilapia. After Streptococcus agalactiae challenge, OnGal-2 expressions were significantly up-regulated in all tested tissues. Meanwhile, the recombinant OnGal-2 (rOnGal-2) protein showed strong agglutinating activities against both Gram-negative bacteria and Gram-positive bacteria. Moreover, rOnGal-2 could promote phagocytosis of macrophages. Taken together, the present study indicated that OnGal-2 might play roles in the immune responses of Nile tilapia against bacterial pathogens.

引用

页码：737 / 743

页数：7

共 36 条

[1] Identification of a novel galectin in Sinonovacula constricta and its role in recognition of Gram-negative bacteria [J].

Bai, Yuqi ;

Niu, Donghong ;

Bai, Yulin ;

Li, Yan ;

Lan, Tianyi ;

Peng, Maoxiao ;

Dong, Zhiguo ;

Li, Jiale .

FISH & SHELLFISH IMMUNOLOGY, 2018, 80 :1-9

[2] A galectin contributes to the innate immune recognition and elimination of pathogens in the freshwater mussel Hyriopsis cumingii [J].

Bai, Zhiyi ;

Zhao, Liting ;

Chen, Xiajun ;

Li, Qingqing ;

Li, Jiale .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2017, 73 :36-45

[3] Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine [J].

Bando, Jennifer K. ;

Liang, Hong-Erh ;

Locksley, Richard M. .

NATURE IMMUNOLOGY, 2015, 16 (02) :153-+

[4] "In vitro" studies on galectin-3 in human natural killer cells [J].

Brittoli, Alvaro ;

Fallarini, Silvia ;

Zhang, Hao ;

Pieters, Roland J. ;

Lombardi, Grazia .

IMMUNOLOGY LETTERS, 2018, 194 :4-12

[5] Galectin-1: a small protein with major functions [J].

Camby, Isabelle ;

Le Mercier, Marie ;

Lefranc, Florence ;

Kiss, Robert .

GLYCOBIOLOGY, 2006, 16 (11) :137R-157R

[6] Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein [J].

Cha, Gui-Hong ;

Liu, Yuan ;

Peng, Ting ;

Huang, Ming-Zhu ;

Xie, Chen-Ying ;

Xiao, Yu-Chao ;

Wang, Wei-Na .

MOLECULAR IMMUNOLOGY, 2015, 67 (02) :325-340

[7] Blood-brain barrier invasion by group B Streptococcus depends upon proper cell-surface anchoring of lipoteichoic acid [J].

Doran, KS ;

Engelson, EJ ;

Khosravi, A ;

Maisey, HC ;

Fedtke, I ;

Equils, O ;

Michelsen, KS ;

Arditi, M ;

Peschel, A ;

Nizet, V .

JOURNAL OF CLINICAL INVESTIGATION, 2005, 115 (09) :2499-2507

[8] Total replacement of fish meal with animal protein sources in Nile tilapia, Oreochromis niloticus (L.), feeds [J].

El-Sayed, AFM .

AQUACULTURE RESEARCH, 1998, 29 (04) :275-280

[9]

Erokhin V. V., 1984, COMP MORPHOFUNCTIONA

[10] Molecular and functional characterization of CD59 from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae [J].

Gan, Zhen ;

Wang, Bei ;

Zhou, Wei ;

Lu, Yishan ;

Zhu, Weiwei ;

Tang, Jufen ;

Jan, JiChang ;

Wu, Zaohe .

FISH & SHELLFISH IMMUNOLOGY, 2015, 44 (01) :50-59

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