Characterization of Galectin-2 from Nile tilapia (Oreochromis niloticus) involved in the immune response to bacterial infection

被引：19
作者：
Niu, Jinzhong [1 ,2 ,3 ]
Huang, Yu [1 ,2 ,3 ,4 ]
Niu, Jimin [1 ,2 ,3 ]
Wang, Zhiwen [1 ,2 ,3 ]
Tang, Jufen [1 ,2 ,3 ,4 ]
Wang, Bei [1 ,2 ,3 ,4 ]
Lu, Yishan [1 ,2 ,3 ,4 ]
Cai, Jia [1 ,2 ,3 ,4 ]
Jian, Jichang [1 ,2 ,3 ,4 ]
机构：
[1] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China
[2] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China
[3] Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China
[4] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2019年 / 87卷
基金：
中国国家自然科学基金;
关键词：
Galectin-2; Nile tilapia; Streptococcus agalactiae; Agglutination; Phagocytosis; Immune system; FUNCTIONAL-CHARACTERIZATION; BINDING LECTIN; HEAD KIDNEY; IN-VITRO; EXPRESSION; IDENTIFICATION; FISH; RECOGNITION; CONTRIBUTES; MACROPHAGES;
D O I：
10.1016/j.fsi.2019.02.026
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
galectin-2 plays important roles in innate and adaptive immunity. In this study, galectin-2 (OnGal 2) was identified from Nile tilapia (Oreochromis niloticus). Its tissue distribution and expression patterns following bacterial infection were also investigated. OnGal-2 is widely distributed in various tissues of healthy tilapia. After Streptococcus agalactiae challenge, OnGal-2 expressions were significantly up-regulated in all tested tissues. Meanwhile, the recombinant OnGal-2 (rOnGal-2) protein showed strong agglutinating activities against both Gram-negative bacteria and Gram-positive bacteria. Moreover, rOnGal-2 could promote phagocytosis of macrophages. Taken together, the present study indicated that OnGal-2 might play roles in the immune responses of Nile tilapia against bacterial pathogens.
引用
收藏
页码：737 / 743
页数：7
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